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目的 探讨癌细胞血管侵润转移机理。方法 采用免疫组织化学方法对 8例直肠腺癌患者的癌周组织和转移淋巴结 ,以及 5例正常人的直肠组织和淋巴细胞间的细胞粘附分子 (ICAM- 1)和核转录因子 κBp6 5(NFκBp6 5 )的表达进行检测。同时还采用地高辛 -碱性磷酸酶标记的寡核苷酸探针 ,用原位杂交技术对 8例直肠腺癌患者的癌周组织和转移淋巴结以及 5例正常人的直肠组织和淋巴结 ICAM- 1基因的 NFκB结合位点进行检测。结果 直肠腺癌患者癌周的淋巴结和癌周直肠组织中的血管内皮细胞都有 ICAM- 1和 NFκBp6 5的表达 ,而在正常人的血管内皮细胞则无 ICAM- 1和 NFκBp6 5的表达 ;直肠腺癌患者癌周淋巴结和癌周直肠组织中的血管内皮细胞核内 ICAM- 1的启动子中存在有 NFκB结合位点。结论 ICAM- 1的转录取决于可诱导的 NFκB蛋白质复合物与 ICAM- 1的 NFκB部位结合。因此 ,调节和控制 NFκB因子的活化能够防止癌细胞的血管转移。
Objective To investigate the mechanism of invasion and metastasis of cancer cells. METHODS: Immunohistochemical method was used in the detection of 8 cases of rectal adenocarcinoma tissues and metastatic lymph nodes, as well as between the rectum tissue and lymphocyte intercellular adhesion molecule (ICAM-1) and nuclear transcription factor κBp6 5 ( The expression of NFκBp6 5 ) was detected. Digoxin-alkaline phosphatase-labeled oligonucleotide probes were also used. In situ hybridization was performed on 8 cases of rectal adenocarcinoma tissues and metastatic lymph nodes, as well as rectal tissues and lymph nodes of 5 normal subjects. - 1 gene NFκB binding sites were detected. RESULTS: The expression of ICAM-1 and NFκBp65 in the lymph nodes around the cancer and the rectal tissues of the rectal adenocarcinoma were positive, but there was no expression of ICAM-1 and NFκBp65 in the normal human vascular endothelial cells. There is an NFκB binding site in the promoter of ICAM-1 in the nucleus of vascular endothelial cells in adenocarcinoma patients with peripheral lymph node and pericancerous tissue. Conclusions The transcription of ICAM-1 depends on the inducible binding of the NFκB protein complex to the NFκB site of ICAM-1. Therefore, regulation and control of activation of NFκB factors can prevent vascular metastasis of cancer cells.