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目的:探讨糖尿病大鼠早期口服中药三七粉对糖尿病大鼠视网膜血流动力学的影响。方法:60只SD大鼠随机平均分成4组,A:正常大鼠生理盐水组;B:正常大鼠三七组;C:糖尿病大鼠生理盐水组;D:糖尿病大鼠三七粉组。C、D组尾静脉注射2%STZ(55 mg/kg)制作糖尿病模型成功后;三七粉治疗组每日按400 mg/kg三七粉灌胃给药一次,生理盐水组每日给予等量的生理盐水灌胃,12周后,应用彩色多普勒能量显像技术检测视网膜中央动脉(CRA)血流的收缩期峰值血流速度(peak systolic velocity,PSV)、舒张期最低流速(end diastolic velocity,EDV)、阻力指数(resistive index,RI)及搏动指数(pulsitive index,PI);心腔采血检测各组大鼠血液的血小板黏附率、血小板聚集率等指标。结果:糖尿病组大鼠视网膜CRA的PSV(17.713±1.43)、EDV(7.601±1.08)较三七治疗组的EDV(12.54±1.27)明显降低(P<0.05),差异有统计学意义;RI(0.569±0.031)、PI(0.848±0.058)升高,但差异无显著统计学意义(P>0.05)。正常大鼠三七干预以后,PSV明显增高,与普通正常大鼠相比差异有统计学意义(P<0.05),而EDV、PI和RI与普通正常大鼠相比无明显差异。糖尿病三七治疗组CRA的PSV、EDV、PI和RI均较正常大鼠高,但差异无统计学意义(P>0.05)。经三七治疗的糖尿病大鼠的血小板黏附率(33.02±2.10)、血小板聚集率(74.83±3.713),与未经三七治疗的糖尿病大鼠血小板黏附率(40.50±3.62)和血小板聚集率(84.00±6.48)相比明显下降(P<0.05),但仍高于正常对照组。三七干预的正常大鼠与未干预的正常大鼠相比,虽然血小板黏附率和血小板聚集率均有所下降,但无明显差异(P>0.05)。结论:三七可降低糖尿病大鼠血小板黏附率、血小板聚集率;改善糖尿病大鼠视网膜中央动脉的血供,对早期糖尿病大鼠视网膜微血管血流动力学具有一定的改善作用。
Objective: To investigate the effects of early oral administration of Panax notoginseng powder on diabetic retinopathy in diabetic rats. Methods: Sixty Sprague-Dawley rats were randomly divided into 4 groups randomly: A: normal saline group; B: normal rat panax notoginseng group; C: diabetic rat normal saline group; D: Panax notoginseng powder group. Group C and group D were injected with 2% STZ (55 mg / kg) into the tail vein to make the model of diabetes mellitus. After the treatment, the group of Panax notoginseng powder was intragastrically administered 400 mg / kg of Panax notoginseng gavage once daily and the saline group was given daily After 12 weeks, the peak systolic velocity (PSV), the minimum diastolic velocity (end diastolic velocity) of the central retinal artery (CRA) blood flow were detected by color Doppler energy imaging. diastolic velocity (EDV), resistive index (RI) and pulsitive index (PI) were measured. Cardiac blood collection was used to detect the blood platelet adhesion rate and platelet aggregation rate in each group. Results: The PSV (17.713 ± 1.43) and EDV (7.601 ± 1.08) of retinal CRA in retina of diabetic rats were significantly lower than those of panax notoginseng (12.54 ± 1.27) (P <0.05), and the difference was statistically significant 0.569 ± 0.031), PI (0.848 ± 0.058), but the difference was not statistically significant (P> 0.05). Compared with normal rats, PSV was significantly increased in normal rats after PSII intervention (P <0.05), while EDV, PI and RI showed no significant difference compared with normal rats. The PSV, EDV, PI and RI of CRA in diabetes mellitus group were higher than those in normal rats, but the difference was not statistically significant (P> 0.05). The platelet adhesion rate (33.02 ± 2.10), platelet aggregation rate (74.83 ± 3.713), and platelet adhesion rate (40.50 ± 3.62) and platelet aggregation rate ( 84.00 ± 6.48) (P <0.05), but still higher than the normal control group. Compared with the normal rats without intervention, the normal rats treated with Panax notoginseng had no significant difference in the rate of platelet adhesion and platelet aggregation (P> 0.05). Conclusion: Panax notoginseng can reduce the rate of platelet adhesion and platelet aggregation in diabetic rats and improve the blood supply of the central retinal artery in diabetic rats, which can improve the hemodynamics of retinal microvascular in diabetic rats.