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采用RAPD-PCR技术,研究了取食6种不同十字花科寄主植物第4、9、15代小菜蛾Plutella xylostella(L.)种群的DNA多态性。通过17条引物的重复PCR扩增实验,利用NTsys软件对电泳条带统计分析,构建了遗传距离矩阵和聚类分析图谱。结果表明:取食芥蓝的小菜蛾种群与其它5种寄主种群遗传距离较远(0.6523～0.8246),独立聚为一枝;取食萝卜的小菜蛾种群遗传距离与甘蓝上种群较近(0.3443),聚为一枝,并且与白菜、芥菜和菜心上小菜蛾种群遗传距离较近,逐次聚为一枝,芥蓝、萝卜及甘蓝寄主种群随代次的增多种群分化出现稳定规律性。各寄主种群间遗传距离平均值和范围、多态性条带占总条带数目的比例随代次增多而增大,说明种群分化程度随代次增多而加深。
The DNA polymorphism of Plutella xylostella (L.) populations on the 4th, 9th, 15th generations of diamondback moth (Plutella xylostella) fed on 6 different cruciferous host plants was studied using RAPD-PCR technique. Through the repeated PCR amplification experiments of 17 primers, using the NTsys software for the statistical analysis of the electrophoresis bands, the genetic distance matrix and the cluster analysis map were constructed. The results showed that the populations of P. xylostella fed on Chinese cabbage were distant from the other five host populations (0.6523-0.8246), and clustered independently. The genetic distance to diamondback moth (Plutella xylostella) fed on radish was close (0.3443) , And clustered together with the diamondback cabbage, mustard and Brassica campestris populations close genetic distance, one by one into a branch, Chinese kale, radish and cabbage host population with the generation of increased population stability and stability. The average value and range of genetic distances among host populations, the proportion of polymorphic bands to the total number of bands increased with the increase of generations, indicating that the degree of population differentiation deepened with the increase of generations.