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目的:观察牛蒡子苷对db/db自发型糖尿病小鼠的降血糖作用及其潜在的作用机制。方法:40只db/db小鼠随机分为5组:模型对照组,牛蒡子苷75,150,300 mg·kg-1组和二甲双胍300 mg·kg-1组,同时设置db/m小鼠空白对照组,灌胃给予相应药物或溶媒,连续4周。给药3周后,进行口服糖耐量试验。4周给药结束后,小鼠禁食12 h,称体质量,检测空腹血糖值(FBG),处死动物,取血清,检测胰岛素(INS)、糖化血清蛋白(GSP)、三酰甘油(TG)、总胆固醇(TC)和脂联素(APN)含量。结果:与模型对照组比较,牛蒡子苷中、高剂量能显著降低db/db小鼠FBG、INS、GSP、TG、TC和APN的血清浓度,改善小鼠糖耐量(P<0.05或0.01)。结论:牛蒡子苷能显著改善db/db小鼠糖脂代紊乱,减轻胰岛素抵抗,其作用机制可能与上调脂联素表达有关。
Objective: To observe the hypoglycemic effect of arctiin on db / db spontaneous diabetic mice and its potential mechanism. Methods: Forty db / db mice were randomly divided into 5 groups: model control group, 75,150 and 300 mg · kg -1 arctigenin group and 300 mg · kg -1 metformin group. At the same time, db / m mice blank control group, Gavage given the appropriate drug or vehicle for 4 weeks. Three weeks after administration, an oral glucose tolerance test was conducted. After 4 weeks, the mice were fasted for 12 h, and their body weight, fasting blood glucose (FBG), animals sacrificed, serum, insulin, glycosylated serum protein (GSP), triglyceride ), Total cholesterol (TC) and adiponectin (APN) content. Results: Compared with the model control group, the high dose of arctiin significantly decreased the serum concentrations of FBG, INS, GSP, TG, TC and APN in db / db mice and improved the glucose tolerance of mice (P <0.05 or 0.01) . Conclusion: Arctiin can significantly improve glycometabolism and insulin resistance in db / db mice, and its mechanism may be related to the up - regulation of adiponectin expression.