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采用国内合成的８－氯腺苷经体内、外实验研究，结果表明，用１００ｍｇ·ｋｇ￣（－１）·ｄ￣（－１）×７剂量，可使小鼠肝癌实体瘤Ｈ＿２２抑制率达７１．７％±１３．３％腹腔注射（ｉｐ）和６６．１％±４．４６％静脉注射（ｉｖ），使白血病Ｌ１２１０荷瘤小鼠的生命延长率达１２４．Ｏ％±２２．１％（ｉｐ）和１０４．２％±２０．１％（ｉｖ）。体外实验显示，８－氯腺苷对人白血病细胞系ＨＬ－６０和Ｋ＿５６２以及人胃癌细胞系ＭＧＣ８Ｏ－３均有明显抑制生长作用，其ＩＣ＿５０值分别为：１．８μｍｏｌ／Ｌ、４．２μｍｏｌ／Ｌ和１．５６μｍｏｌ／Ｌ。该药采用腹腔一次注射测定小鼠的ＬＤ＿５０为：１０２５．０±５２．４ｍｇ／ｋｇ；大鼠的ＬＤ＿５０为：７９３．４±７０．７ｍｇ／ｋｇ，表明其急性毒性很低。
The results showed that the inhibitory rate of H_22 in mouse hepatoma solid tumor was up to 100 mg · kg -1 (-1) × 7 with the domestic synthesis of 8-chloro-adenosine in vitro and in vivo. 71.7% ± 13.3% ip and 66.1% ± 4.46% intravenous injection (iv). The leukemia L1210 tumor-bearing mice had a lifesaving rate of 124. O% ± 22.1% (ip) and 104.2% ± 20.1% (iv). In vitro experiments showed that 8-chloro-adenosine inhibited the growth of human leukemia cell lines HL-60 and K_562 and human gastric cancer cell line MGC8O-3 with IC50 values of 1.8μmol / L and 4.2μmol / L and 1.56 μmol / L. The drug LD50 measured by intraperitoneal injection of mice: 1025.0 ± 52.4mg / kg; LD_50 rat: 793.4 ± 70.7mg / kg, indicating that its acute toxicity is very low.