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目的对我国南北两城市530份乙型肝炎(乙肝)病毒(HBV)进行血清型和基因型分型,以了解HBV基因型和血清型分布的特点和差异。方法 对黑龙江省哈尔滨市和广东省廉江市530份乙肝病毒表面抗原(HBsAg)阳性血清进行HBV DNA基因扩增,并对扩增产物直接测序,分析HBV血清亚型和基因型。结果 哈尔滨和廉江市HBV血清型以adrq为最多,分别为87.2％和73.5％,其次为adw2,分别为12.0％和25.7％,其分布差异有非常显著意义(P<0.001);两市HBV基因型均以C型为主,分别为87.8％,73.2％,其次为B型,分别为12.2％和26.1％,其分布差异有非常显著意义(P<0.001),在廉江市仅有1例D型,1例B、C混合型。结论我国南北两城市HBV血清亚型和基因型的构成较为单纯,均只有两个类型,其比例构成差异有非常显著意义。
Objective To analyze the serotypes and genotypes of 530 hepatitis B virus (HBV) strains in two cities of north and south China to understand the characteristics and differences of HBV genotype and serotype distribution. Methods HBV DNA was amplified from 530 hepatitis B virus surface antigen (HBsAg) positive sera in Harbin, Heilongjiang Province and Lianjiang City, Guangdong Province. HBV DNA was sequenced and the HBV serosubtypes and genotypes were analyzed. Results The HBV serotypes of Harbin and Lianjiang had the highest adrq (87.2% and 73.5%, respectively), followed by adw2 (12.0% and 25.7% respectively), with significant differences in distribution (P <0.001) The genotypes were mainly C genotype (87.8%, 73.2%, respectively), followed by type B (12.2% and 26.1%, respectively), with significant differences in distribution (P <0.001) Case D type, 1 case B, C mixed type. Conclusion The HBV serosubtypes and genotypes in the two cities of North and South China are relatively simple, with only two types. The proportions of HBV serotypes and genotypes are very significant.