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无论在harpin_(Pss)之前、同时、还是之后向烟草植株或悬浮培养系加阴离子通道的抑制剂DIDS(4,4’-diisothiocyanatostilbene-2,2’-disulfonic acid)或AgC(anthracene-9-carboxylic acid),都可以抑制harpin_(Pss)诱导的烟草植株过敏反应和悬浮细胞的活性氧的释放及胞外碱性化。DIDS和A9C还可以抑制harpin_(Pss)诱导的Ca~(2+)内流。而且DIDS的抑制效率比A9C高。推测质膜上的阴离子通道对钙离子通道有着正调节作用,harpin_(Pss)通过阴离子通道和钙离子通道介导的信号传导途径,调节胞内Ca~(2+)浓度,从而启动这些防卫反应。
Both the DIDS (4,4’-diisothiocyanatostilbene-2,2’-disulfonic acid) and the anthracene-9-carboxylic acid (DID) inhibitors of anionic channels were added to tobacco plants or suspension cultures either before harumi pss, all inhibited the harpin (Pss) -induced allergic reaction in tobacco plants and the release of active oxygen and extracellular basalisation in suspension cells. DIDS and A9C also inhibited harpin (Pss) -induced Ca 2+ influx. And DIDS inhibition efficiency higher than A9C. It is hypothesized that the anion channels on the plasma membrane have a positive regulatory effect on the calcium channel. Harpin Pss, which regulates intracellular Ca 2+ concentration through anion channels and calcium channel-mediated signal transduction pathways, initiates these defense responses .