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以南海贝类翡翠贻贝全脏器为原料提取得到糖胺聚糖粗品,经蛋白质等电点沉淀法处理后得到糖胺聚糖精制品(RG),糖胺聚糖含量52.3%。应用MTT法检测糖胺聚糖精制品对人鼻咽癌细胞CNE-2Z体外抗肿瘤活性。结果显示:RG不同剂量在作用不同时间后的抑瘤效果不同,其中200μg/mL的RG作用72h抑瘤率达到最高值为29.3%。RG与5-Fu合用的抑瘤效果,随着药物作用时间的延长,两者由协同作用变为单纯相加作用。
The crude glycosaminoglycan was extracted from the whole organ of the South China Sea shellfish Jade Mytilus edulis. The glycosaminoglycan product (RG) was obtained after the isoelectric point precipitation of the protein. The glycosaminoglycan content was 52.3%. MTT assay was used to detect the in vitro antitumor activity of glycosaminoglycans on human nasopharyngeal carcinoma cell CNE-2Z. The results showed that different doses of RG had different antitumor effects after different times of action. Among them, the tumor inhibition rate of 200μg/mL RG for 72h reached the highest value of 29.3%. The inhibitory effect of RG and 5-Fu combined with the prolongation of the drug action time, the two from the synergistic effect to a simple additive effect.