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目的:制备含钙纳米薄片膜层修饰的双酸酸蚀钛表面并评价其对成骨细胞行为的影响。方法:通过双酸酸蚀和氢氧化钙/双氧水混合溶液水热处理,制备2种含钙纳米薄片膜层修饰的双酸酸蚀钛表面(1 h、6 h处理组)。以大颗粒喷砂酸蚀(SLA)钛表面为对照组,2种含钙纳米薄片膜层修饰的双酸酸蚀钛表面为实验组,观察分析不同钛表面的微形貌和表面元素组成;将MC3T3-E1成骨细胞接种于各组试件表面,研究不同钛表面对成骨细胞生物学行为的影响。结果 :在双酸酸蚀钛表面制备形成2种形貌均一的纳米薄片膜层结构,均含有微量钙元素。相比于SLA钛表面,2种新型钛表面均有利于MC3T3-E1成骨细胞的黏附和增殖,显著增强成骨细胞的碱性磷酸酶活性,并上调成骨相关基因的表达。其中,1 h处理组性能更优。结论:双酸酸蚀钛表面复合含钙纳米薄片膜层能有效促进成骨细胞的黏附、增殖和分化。
OBJECTIVE: To prepare double-acid etching titanium surface modified with calcium-containing nanosheet film and evaluate its effect on osteoblast behavior. Methods: Two types of acid-etched titanium surfaces modified with calcium-containing nanosheet films were prepared by double acid etching and hydrothermal treatment with calcium hydroxide / hydrogen peroxide mixed solution (1 h, 6 h treatment group). The surface of double-acid etching titania modified with two kinds of calcium-containing nanosheet films was used as experimental group, and the micro-topography and surface elemental composition of different titanium surfaces were observed and analyzed. The MC3T3-E1 osteoblasts were inoculated on the surface of each group to study the effect of different titanium surfaces on the biological behavior of osteoblasts. Results: Two kinds of nanolayer films with uniform morphology were formed on the surface of double-acid etched titania. All of them contained trace calcium. Compared with the surface of SLA titanium, the two novel titanium surfaces are conducive to MC3T3-E1 osteoblasts adhesion and proliferation, significantly increased alkaline phosphatase activity of osteoblasts, and up-regulate osteogenic related gene expression. Among them, 1 h treatment group performed better. CONCLUSION: The double-acid etching of titanium surface with calcium-containing nanosheets can effectively promote the adhesion, proliferation and differentiation of osteoblasts.