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目的:研究Galectin-3在4种不同Duke分期的大肠癌细胞系中的差异表达,探讨与大肠癌病理特征的关系。方法:体外培养SW1116、SW480、SW620和LoVo4种不同病理分期大肠癌细胞株,以NIH3T3人类成纤维细胞及肾癌T淋巴细胞作为阴性对照组,胃癌SGL7901细胞作为阳性对照组,用免疫细胞化学方法对Galectin-3进行细胞内定位,RT-PCR方法研究Galectin-3在mRNA水平的表达;蛋白质印迹法研究在蛋白水平的表达。结果:Galectin-3主要表达在大肠癌细胞质中。在Duke分期依次为A到D期的SW1116、SW480、SW620和LoVo4种细胞系中,免疫染色强度逐步增强;在4种不同Duke分期的大肠癌细胞中,Galectin-3的表达水平在mRNA水平和蛋白水平均显示在A期的SW1116中表达较弱,在D期的LoVo中表达最强。组间比较,F值分别为268138.2,942152.2,各组间两两比较,P均<0.05,差异有统计学意义。两者依次增强,各组间比较差异均有统计学意义,P<0.05。结论:Galectin-3主要表达在大肠癌细胞质上,从基因和蛋白水平均表明,Ga-lectin-3在4种大肠癌细胞中均有表达,其中在A期的SW1116细胞中表达较弱,在D期的LoVo表达最强。这提示Galectin-3在大肠癌中的表达可作为大肠癌预后的一个指标。
OBJECTIVE: To study the differential expression of Galectin-3 in four kinds of colorectal cancer cell lines with different Duke staging and to explore the relationship with the pathological features of colorectal cancer. METHODS: Four colon cancer cell lines with different pathological stages of SW1116, SW480, SW620 and LoVo were cultured in vitro. NIH3T3 human fibroblasts and renal T lymphocytes were used as negative control group. Gastric cancer SGL7901 cells were used as positive control group. Immunocytochemistry Galectin-3 was intracellularly localized, the expression of Galectin-3 at the mRNA level was studied by RT-PCR, and the protein expression at the protein level by Western blotting. Results: Galectin-3 was mainly expressed in the cytoplasm of colorectal cancer. The intensity of immunostaining was gradually increased in SW1116, SW480, SW620 and LoVo4 cell lines with Dukes stages A to D, and the expression levels of Galectin-3 in four different Duke stages of colorectal cancer cells were significantly higher at mRNA level and The protein levels showed weak expression in SW1116 in stage A and the strongest in LoVo in stage D. Between groups, the F values were 268138.2 and 942152.2, respectively, and the differences between groups were significant (P <0.05). The difference was statistically significant. The two groups in turn increased, the differences between the groups were statistically significant, P <0.05. CONCLUSIONS: Galectin-3 is mainly expressed in the cytoplasm of colorectal cancer. Both gene and protein levels indicate that Ga-lectin-3 is expressed in all four colorectal cancer cells. The expression of Galectin-3 is weak in SW1116 cells in stage A, D LoVo expression of the strongest. This suggests that Galectin-3 expression in colorectal cancer can be used as an indicator of the prognosis of colorectal cancer.