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所谓树脂问题是指木材和其他的木素纤维素材料中的亲脂性抽出物在制浆造纸过程中产生的不利冲击。本文采用漆酶/介体系统进行树脂的生物控制,并对漆酶催化处理的可行性进行评价。为了这一目的,我们以1-羟基苯并三唑作为氧化还原介体,采用从担子菌类中的Pycnoporus硃红菌素中提取出的高氧化还原电位的漆酶来处理三种不同类型的浆,三种浆分别代表三种不同的原材料制浆过程,它们是桉木硫酸盐浆、云杉热磨机械浆和亚麻苏打-蒽醌浆,然后再用气相色谱和气相色谱-质谱联用分析酶催化处理的浆中的亲脂性抽出物,研究结果表明,漆酶/介体系统处理法可以完全或者大部分地除去树脂引起的亲脂性化合物存在于不同浆中的问题,它包括(1)桉纸浆中游离和共轭的谷甾醇;(2)云杉浆中的树脂酸、甾醇酯和甘油三酯;(3)亚麻浆中的甾醇和脂肪醇。浆中甾醇的氧化过程中会发现有不同量的游离和共轭的7-氧甾醇作为中间产物生成。因此,用漆酶/介体系统处理阔叶木、针叶木和非木材植物的纸浆,被认为是一种去除树脂产生的亲脂性化合物的有效方法。
The so-called resin problem refers to the negative impact that lipophilic extracts from wood and other lignocellulosic materials have in the pulping and papermaking process. In this paper, laccase / mediator system was used to control the bioactivity of resin, and the feasibility of laccase catalytic treatment was evaluated. For this purpose we used 1-hydroxybenzotriazole as a redox mediator to treat three different types of laccases using the high redox potential extracted from Pycnoporus brucei in basidiomycetes Pulp and three slurries represent three different raw material pulping processes, respectively, eucalyptus kraft pulp, spruce thermomechanical pulp and flax soda-anthraquinone pulp, followed by gas chromatography and gas chromatography-mass spectrometry The analysis of the lipophilic extracts in the enzymatically treated pulp showed that the laccase / mediator system process could completely or largely eliminate the problem of resin-induced lipophilic compounds present in different slurries including (1 ) Free and conjugated sitosterol in eucalyptus pulp; (2) Resin acids, sterol esters and triglycerides in spruce pulp; (3) Sterols and fatty alcohols in linen flax pulp. Oxidation of the sterol in the pulp reveals different amounts of free and conjugated 7-oxysterol as intermediates. Therefore, the treatment of pulps of hardwood, softwood and non-woody plants with a laccase / mediator system is considered to be an effective method of removing resin-derived lipophilic compounds.