论文部分内容阅读
目的提高二益丸的质量标准。方法采用TLC法对白芷、当归进行定性鉴别,采用HPLC法测定欧前胡素的含量,色谱柱为Kromasil 100-5 C_(18),流动相为乙腈-水(55∶45),检测波长:248 nm,流速:1.0 ml·min~(-1),柱温:35℃,进样量:10μl。结果 TLC法中,在与对照品或对照药材相应的位置上显相同颜色的斑点,阴性无干扰;欧前胡素在2.91~58.11μg·ml~(-1)范围内呈良好的线性关系,r=1.00,平均回收率为101.64%,RSD为1.17%(n=6)。结论本方法操作简便、结果准确,可用于二益丸的质量控制。
Purpose To improve the quality standard of Er Yi Wan. Methods TLC and HPLC were used to identify the content of imperatorin. The chromatographic column was Kromasil 100-5 C 18 with the mobile phase of acetonitrile-water (55:45). The detection wavelength was: 248 nm, flow rate: 1.0 ml · min -1, column temperature: 35 ℃, injection volume: 10μl. Results In the TLC method, the same color spots and negative spots were found on the corresponding sites of reference substance or reference drug. There was a good linear relationship between imperatorin and 2.91 ~ 58.11μg · ml ~ (-1) r = 1.00, the average recovery was 101.64%, RSD was 1.17% (n = 6). Conclusion The method is simple, accurate and can be used for the quality control of Er-Yi-Wan.