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Herbal medicines have been attracting intensive attention with the increasing of health care.The screening and analysis of bioactive components in medicinal plants are very important for ensuring their efficacy,safety as well as quality.A conventional procedure for finding bioactive components is the chemical separation followed by pharmacological screening,or bioassay guided separation.In this presentation,chromatographic methods coupled with online bioassay developed in our lab were introduced for discovery of bioactive components from Chinese herbs.