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为检测神经母细胞瘤 (NB) 1号染色体短臂杂合性缺失 (1pLOH) ,并观察(1pLOH)与临床预后的关系 ,应用荧光原位杂交技术 (FISH)———用非放射性物质标记核酸探针 ,根据核酸杂交原理 ,在间期核和染色体上检测特异性DNA序列的一项新技术 ,检测了 2 0例神经母细胞瘤患者的 1pLOH ,结果 6例有 1pLOH ,占 30 % ,并观察到 1pLOH大多发生在年龄大于 2岁的Ⅲ期 /Ⅳ期的神经母细胞瘤患者中。结果表明 :1 .神经母细胞瘤在 1p36 .2— 36 .3区域内有杂合性缺失 ;2 .1pLOH与NB分期密切相关 (P <0 .0 1 ) ;3.1pLOH与病人确诊时的年龄密切相关 (P <0 .0 5 ) ;4.1pLOH与NB预后密切相关 (P <0 .0 1 )。
To detect the short arm heterozygosity (1pLOH) on chromosome 1 of neuroblastoma (NB) and observe the relationship between (1pLOH) and clinical prognosis, we used fluorescent in situ hybridization (FISH) - labeled with non-radioactive substances Nucleic acid probe, according to the principle of nucleic acid hybridization, in the interphase nucleus and chromosome detection of specific DNA sequence of a new technology, 20 cases of neuroblastoma patients detected 1pLOH, the results of 6 cases 1pLOH, accounting for 30% 1pLOH was observed mostly in patients with stage III / IV neuroblastoma older than 2 years of age. The results showed that: 1.Neuroblastoma has heterozygous deletion in the region of 1p36.2-36.3; 2.lpLOH is closely related to the stage of NB (P <0.01); 3.1pLOH is not related to the age at diagnosis (P <0.05). 4.1pLOH was closely related to the prognosis of NB (P <0.01).