【摘 要】
：
This study examined the effects of clinically relevant concentrations of isoflurane on the amplitude of NMDA receptor current (INMDA) and the expression of cyto
【机 构】
：
Department of Anesthesiology, Tongji Hospital, Tongji Medical College, Huazhong University of Scienc
【出 处】
：
华中科技大学学报（医学）（英德文版）
论文部分内容阅读
This study examined the effects of clinically relevant concentrations of isoflurane on the amplitude of NMDA receptor current (INMDA) and the expression of cytochrome C in cultured developing rat hippocampal neurons.The hippocampi were dissected from newb Sprague-Dawley rats.Hippocampal neurons were primarily cultured for 5 days and then treated with different concentrations of isoflurane [(0.25,0.5,0.75,1 minimum alveolar concentration (MAC))].The peak of INMDA was recorded by means of the whole cell patch clamp technique.The cytochrome C level was detected by West blotting and quantitative real-time PCR.Our results showed that isoflurane (0.25,0.5,0.75 and 1 MAC) potentiated the amplitude of INMDA by (116±8.8)％,(122±11.7)％,(135±14.3)％ and (132±14.6)％,respectively,and isoflurane increased the mRNA expression of cytochrome C in a concentration-dependent manner.The cytochrome C mRNA expression reached a maximum after 0.5 MAC isoflurane stimulation for 6 h (P＜0.05).It was concluded that isoflurane enhances the expression of cytochrome C in cultured rat hippocampal neurons,which may be mediated by facilitation of NMDA receptor.
其他文献
乃黄帝与岐伯上穷天纪,下极地理,远取诸物,近取诸身,更相问难而成.其意博,其理奥,其趣深,历代医家莫不刻意精研,探微索隐.笔者亦从师读之,以期识契真要.讨论了因风邪侵人人体
We present a 69-year-old woman with a duodenal obstruction after successful selective transcatheter arterial embolization (TAE) for a duodenal diverticular hemo
The aim of this study was to investigate the effect of Paris saponin Ⅰ (PS Ⅰ ) on human gastric carcinoma cell growth and apoptosis and to explore the potenti
目的:探讨新生儿科医院感染干预措施对降低新生儿科医院感染发病率,减少住院天数的效果.方法:2012年9月至2013年2月新生儿科所有病例纳入对照组进行目标性监测,2013年3月至20
糖尿病治疗的基础是寻求生活方式干预.缺乏体力活动和过量进食等不健康的生活方式会引发并促进大多数2型糖尿病的发病.在美国,几乎所有年龄段和种群糖尿病发病率上升与超重和
“我从小就是孤儿,当过保姆,当过童养媳,我没有母亲,从小我连爹也没有叫过一声,妈也没有叫过一声,只有共产党是我的母亲,我—定要好好回报她.我也想更好地回报这个社会,可是
原发性痛经(PD)属于妇科临床常见疾病,其病因病机至今不完全明确,内分泌失调是原发性痛经发生的主要原因.近年来研究证实PD存在免疫功能失调而致病的可能,而淋巴Th1/Th2型细
目的探讨KRAS基因突变与结直肠癌患者临床病理特征及预后的关系。方法采用回顾性病例对照研究方法。收集2007年1月至2011年7月同济大学附属杨浦医院收治的315例行结直肠癌手术患者的临床病理资料。采用高通量测序技术检测结直肠癌手术切除标本石蜡切片KRAS基因突变情况。分析指标：(1)KRAS基因检测情况。(2)KRAS基因突变状态与结直肠癌患者临床病理因素的关系。(3)随访和生存情况。(4)结直
目的 评价置管溶栓联合腔内成形术治疗急性下肢动脉血栓形成的疗效.方法 2012年1月至2016年7月,对42例下肢动脉血栓形成的患者,先急诊进行置管溶栓,3 d后造影联合腔内成形术