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目的:探讨不同氨溴索给药方式对大鼠机械通气肺部炎性因子释放及肺组织湿/干比值的影响。方法:将45只健康SD雄性大鼠随机分成静脉组、雾化组和对照组,各15只。对照组:大鼠麻醉后行气管切开插管,并行单纯机械通气4h;静脉组:大鼠麻醉后从尾静脉泵入氨溴索,气管切开插管后行机械通气4h;雾化组:大鼠麻醉后行气管切开插管,通过自制装置氧气驱动雾化吸入氨溴索,并行机械通气4 h。各组机械通气后放血处死大鼠,取肺组织,计算肺湿/干重比(W/D)。收集支气管灌洗液(BALF)并检测TNF-α,IL-1β和IL-6浓度。结果:与对照组比较,静脉组和雾化组大鼠支气管灌洗液中TNF-α,IL-1β和IL-6的浓度明显降低(P<0.05),但此两组间无显著性差异(P>0.05);与对照组比较,静脉组和雾化组大鼠肺组织W/D比也明显降低(P<0.05),但雾化组大鼠肺组织W/D比明显低于静脉组大鼠(P<0.05)。结论:氨溴索静脉给予和雾化给予都能显著降低大鼠机械通气时肺内炎性因子水平和肺W/D比,而且雾化给予较静脉给予更能减轻大鼠肺组织的W/D比。
Objective: To investigate the effects of different ambroxol on pulmonary inflammatory factor release and lung wet / dry ratio in rats with mechanical ventilation. Methods: Forty-five healthy SD male rats were randomly divided into intravenous group, nebulized group and control group, 15 rats each. Control group: rats were anesthetized by endotracheal intubation, simple mechanical ventilation and parallel 4h; venous group: rats were anesthetized ambroxol pump from the tail vein, tracheotomy intubation after mechanical ventilation 4h; atomization group Rats were anesthetized and tracheotomized. Ambroxol was inhaled aerosolized by a homemade device and mechanically ventilated for 4 h. Rats in each group were sacrificed by mechanical ventilation and lungs were taken out to calculate the lung wet / dry weight ratio (W / D). Bronchoalveolar lavage fluid (BALF) was collected and the concentrations of TNF-α, IL-1β and IL-6 were measured. Results: Compared with the control group, the concentrations of TNF-α, IL-1β and IL-6 in the bronchoalveolar lavages of the venous group and the atomized group were significantly decreased (P <0.05), but there was no significant difference between the two groups (P> 0.05). Compared with the control group, the W / D ratio of pulmonary tissue in the venous group and the nebulized group was also decreased significantly (P <0.05), but the W / D ratio in the lung tissue of the atomized group was significantly lower than that in the control group Group rats (P <0.05). CONCLUSION: Both intravenous administration of ambroxol and nebulization can significantly reduce the level of pulmonary inflammatory cytokines and lung W / D ratio in rats with mechanical ventilation, and that the nebulized rats administered intravenously can attenuate the W / D ratio.