为了鉴定异常血红蛋白的类型,需要对血红蛋白的异常肽链进行结构分析。本文介绍参照Clegg法修改的一种血红蛋白结构分析技术——肽链的指纹分析(肽链酶解产物的高压电泳-层析双向分离技术),并应用这种技术对在我国萍乡发现的血红蛋白Leiden(α_2β_2~(Leiden))和在广州发现的血红蛋白E(α_2β_2~E)进行结构分析。方法一、试剂 (一) 羧甲基纤维素(国产或进口均可)。 (二) 葡聚糖凝胶(Sophadox G25,Pharmacia)。 In order to identify the type of abnormal hemoglobin, structural analysis of abnormal hemoglobin peptide chains is required. This article introduces a kind of hemoglobin structure analysis technology which is modified by Clegg law - the fingerprinting of peptide chain (high pressure electrophoresis and chromatographic bi-directional separation technology of peptide hydrolyzate), and applies this technique to detect the hemoglobin Leiden (α_2β_2 ~ (Leiden)) and hemoglobin E (α_2β_2 ~ E) found in Guangzhou. Method One, reagents (a) carboxymethyl cellulose (domestic or imported can be). (B) Sephadex (Sophadox G25, Pharmacia).