多参数流式细胞术(FCM)检测白血病微小残留病(MRD)具有快速、敏感、可定量的优势。联合抗原受体基因重排的聚合酶链反应检测,可检测几乎所有儿童急性淋巴细胞白血病(ALL)的MRD,可评估早期治疗反应,预示复发,实施个体化治疗,提高生存质量。本文重点介绍多参数FCM检测MRD的原理、优缺点及临床意义。 Multi-parameter flow cytometry (FCM) detection of leukemia minimal residual disease (MRD) with fast, sensitive, quantifiable advantages. Combined with antigen receptor gene rearrangement polymerase chain reaction detection can detect MRD in almost all children with acute lymphoblastic leukemia (ALL), can assess the early response to treatment, indicating recurrence, the implementation of individualized treatment, and improve the quality of life. This article focuses on the principle, advantages and disadvantages, and clinical implications of MRM with multi-parameter FCM.