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目的 改进大鼠大脑中动脉梗塞法 ,建立更接近于临床缺血性脑卒中及其再灌注的可靠模型 ,并观察其与血清 C反应蛋白变化的关系。 方法 沿大鼠右颈内动脉插入长 2 .1～ 2 .3cm直径 0 .2 0 5 mm的单股尼龙丝 ,直达大脑中动脉起始部开口 ,阻断其血流 ,观察大鼠神经病学改变及脑组织形态学变化 ,并测定血清 C反应蛋白含量。 结果 术后大鼠表现特殊体态及典型追尾征 ,6h大脑中动脉供血区出现缺血性外观 ( TTC染色 )及相应组织学变化 ( HE染色 ) ,模型成功率近95 % ( 71/75 ) ;并伴血清 C反应蛋白上升 ( 15 .9% ,n=2 0 ,P<0 .0 5 )。 结论 本方法既可构成缺血性病灶 ,又能形成再灌注模型 ,具有可逆性重复操作的特点 ;其神经病学与形态学的特殊表现及血清 C反应蛋白的升高 ,可作为鉴定模型可靠性的依据。
Objective To improve the model of middle cerebral artery occlusion in rats and establish a more reliable model of clinical ischemic stroke and its reperfusion, and to observe its relationship with the changes of serum C - reactive protein. Methods A single nylon filament with a length of 2.1-2.3 cm and a diameter of 0.225 mm was inserted into the right internal carotid artery of rats to reach the opening of the middle part of the middle cerebral artery to block the blood flow and observe the neurological Changes and changes of brain tissue morphology, and determination of serum C-reactive protein content. Results After operation, the rats presented with special body posture and typical rear - end syndrome. The ischemic appearance (TTC staining) and corresponding histological changes (HE staining) were observed in the middle cerebral artery blood supply area 6 hours after operation. The model success rate was nearly 95% (71/75). And with serum C-reactive protein increased (15.9%, n = 20, P <0. 05). Conclusion This method not only can form ischemic lesions, but also can form a reperfusion model with the characteristics of reversible repetitive operation; its special manifestations of neurology and morphology and elevated serum C-reactive protein can be used to identify the reliability of the model The basis.