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目的:探讨成神经分化的骨髓间充质干细胞(BM-SCs)向C6细胞条件培养基和SDF-1α的趋化性迁移。方法:采用碱性成纤维生长因子(bFGF)、丁羟基茴香醚(BHA)、二甲基亚砜(DMSO)联合诱导剂对BMSCs进行诱导分化,通过免疫荧光染色检测诱导的细胞表达神经前体细胞标志物Nes-tin、β-III-Tubulin和NSE的情况。运用Dunn chamber研究成神经分化的BMSCs向胶质瘤和SDF-1α的迁移。结果:BM-SCs能诱导分化成神经元样细胞,而对照组的BMSCs形态无变化。Dunn chamber分析显示,C6细胞条件培养基组和SDF-1α组诱导细胞的迁移速率和迁移效率明显高于对照组,表明C6细胞条件培养基和SDF-1α对BMSCs具有趋化作用,单个细胞迁移轨迹实验也证实了这一结果。此外,分化不同状态的BMSCs向C6细胞条件培养基和SDF-1α的趋化程度也不同。结论:BMSCs的定向迁移与分化状态密切相关。
Objective: To investigate the chemotactic migration of mesenchymal stem cells (BM-SCs) differentiated into C6 cells and SDF-1α. Methods: BMSCs were induced to differentiate by basic fibroblast growth factor (bFGF), butylated hydroxyanisole (BHA) and dimethyl sulfoxide (DMSO), and the expression of neural precursor was detected by immunofluorescence staining Cell markers Nes-tin, β-III-Tubulin and NSE. Dunn chamber was used to study the migration of neuroblastic BMSCs into glioma and SDF-1α. Results: BM-SCs could differentiate into neuron-like cells, while the control group did not change the morphology of BMSCs. Dunn chamber analysis showed that the migration rate and migration efficiency induced by C6 cell conditioned medium and SDF-1α group were significantly higher than that of the control group, indicating that C6 cell conditioned medium and SDF-1α had a chemotactic effect on BMSCs and a single cell migration Trajectory experiment also confirmed this result. In addition, the differentiation of different states of BMSCs to C6 cell conditioned medium and SDF-1α chemotactic degree is also different. Conclusion: The directional migration of BMSCs is closely related to the differentiation status.