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对于毛细管等速电泳中的电渗,前人已有研究。但在毛细管区带电泳中电渗对分离效率的影响仍有待探讨。本工作测量了在内径0.5及0.3mm毛细管中的电渗流,试验了不同电解质浓度,不同温度和不同pH等条件,由电渗流计算的Zeta电位为-15至-35mV.提高浓度,降低pH或温度可以抑制电渗。加入0.2％HPMC则使电渗为零。在一般的优化条件下(浓度≥0.05M,加有0.2％以上的添加剂),毛细管区带电泳电渗是微不足道的,在大孔径(0.5mm内径)毛细管中尤其如此。因此,进行电泳效率的理论分析时,忽略电渗是可以允许的,这将使数学处理简化。
For the capillary isokinetic electrophoresis, the predecessor has been studied. However, the effect of electroosmosis on the separation efficiency in capillary zone electrophoresis still needs to be explored. In this work, the electroosmotic flow in 0.5 and 0.3 mm ID capillary tubes was measured. The Zeta potential calculated by EO was -15 to -35 mV under different electrolyte concentrations, different temperatures and different pH conditions. Increasing the concentration, decreasing the pH or Temperature can inhibit electroosmosis. Adding 0.2% HPMC resulted in zero electroosmosis. Capillary zone electrophoresis is insignificant under typical optimization conditions (concentration> 0.05 M plus 0.2% additive), especially in large pore (0.5 mm id) capillaries. Therefore, in the theoretical analysis of electrophoresis efficiency, it is permissible to ignore the electroosmosis, which will simplify the mathematical processing.