Early blood-brain barrier(BBB)disturbance contributes to many different neurological diseases including Alzheimer's disease,Parkinson's disease and stroke,particularly in the etiology and early stages(Abbott et al.,2010).Although supporting barrier function is a potential strategy to radically improve treatment efficacy and disease outcome,ways to achieve this objective remain elusive.Being a sophisticated system consisting of multicellular interactions,better understanding of how individual cell-specific molecular and metabolic changes modulate the unit responses would provide significant insight.In this regard,we have generated severity-related metabolomic databases to reveal fundamental BBB cell-specific processes likely to occur physiologically and during disease(Huang et al.,2020a).This resource provides new opportunities for future clinical applications.