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目的:观察和探讨宫内感染致脑损伤仔鼠脑神经髓鞘改变及丰富康复训练的影响和机制。方法:连续2天对孕第17天的脂多糖(LPS)组孕鼠腹腔注射LPS(450μg/kg.d),制备脑损伤模型;对照组孕鼠腹腔注射等量无菌生理盐水。LPS组仔鼠随机分为非干预组和丰富康复训练组,丰富康复训练组于第2天开始进行干预,用电子显微镜观察d14、d28各组仔鼠脑神经髓鞘厚度情况,并应用体视学技术测量和计算脑神经髓鞘厚度平均值,同时用免疫组化方法检测仔鼠脑皮质区BDNF表达情况。结果:非干预组、丰富康复训练组仔鼠脑神经髓鞘厚度平均值均明显低于对照组,差异有显著性(P<0.01),丰富康复训练组仔鼠脑神经髓鞘厚度平均值高于非干预组,差异有统计学意义(P<0.01);丰富康复训练组仔鼠脑的皮质区BDNF阳性细胞计数明显高于对照组和非干预组,差异有显著性(P<0.01)。结论:(1)宫内感染可致脑损伤仔鼠脑神经髓鞘厚度平均值降低。(2)丰富康复训练可提高脑损伤仔鼠脑神经髓鞘厚度平均值。(3)丰富康复训练可增强脑损伤仔鼠脑组织BDNF阳性表达。
OBJECTIVE: To observe and discuss the effects and mechanisms of cerebral nerve myelin changes and extensive rehabilitation training in neonatal rats with intrauterine infection. Methods: LPS (450μg / kg.d) was intraperitoneally injected into LPS pregnant rats on the 17th day of gestation for 2 consecutive days to prepare a model of brain injury. The control rats were injected intraperitoneally with equal volume of sterile saline. LPS rats were randomly divided into non-intervention group and rehabilitation training group, rich rehabilitation training group began to intervene on the 2nd day, electron microscopy of d14, d28 each group of young rats with myelin sheath thickness, and the application of stereoscopic Learning techniques were used to measure and calculate the average thickness of myelinated nerve cells. Meanwhile, the expression of BDNF in cerebral cortex of rats was detected by immunohistochemistry. Results: The mean values of myelin sheath thickness in the non-intervention group and the enriched rehabilitation training group were significantly lower than those in the control group (P <0.01), and the mean values of the myelin sheath thickness in the enriched rehabilitation training group were high (P <0.01). The counts of BDNF positive cells in cortex in the brain of the enriched rehabilitation training group were significantly higher than those in the control group and non-intervention group (P <0.01). Conclusion: (1) Intrauterine infection can lead to brain damage in rats with decreased mean nerve myelin sheath thickness. (2) Extensive rehabilitation training can improve the average thickness of nerve sheath in the brain of offspring. (3) Extensive rehabilitation training can enhance BDNF expression in brain tissue of neonatal rats with brain injury.