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采用离体叶片接种法,观察不同浓度夏孢子悬浮液对大豆叶片的致病反应。结果表明:各个接种浓度均可引起叶片发病,发病率随接种浓度的升高而增加,夏孢子堆密度随接种浓度升高而增加,夏孢子堆破裂时间随接种浓度的增加而缩短,不同品种的致病反应差异主要表现在病斑出现时间、夏孢子堆密度、夏孢子堆破裂时间和发病率等方面。锈菌浓度对孢子堆大小没有显著影响,采用105个夏孢子/mL的接种浓度可以有效地区分不同品种的抗病性。
The in vitro leaf inoculation method was used to observe the pathogenic reaction of different concentrations of nymph spore suspension on soybean leaves. The results showed that all the inoculation concentrations could cause the incidence of leaves, the incidence increased with the increase of inoculation concentration, the density of uredospore increased with the increase of inoculation concentration, the rupture time of summer spores ruptured with the increase of inoculation concentration, The main differences of pathogenic reaction were the appearance time of the lesion, the density of the summer spore, the rupture time of the summer spore and the morbidity. The concentration of rust had no significant effect on the size of the spore reactor. The inoculation concentration of 105 uospores / mL could effectively distinguish the disease resistance of different varieties.