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目的:建立参芪养心滴丸中哈巴俄苷和肉桂酸的含量测定方法。方法:采用HPLC法;色谱柱为Kromasil-C_(18)柱(200mm×4.6 mm,5μm);流动相为乙腈-1%醋酸(28:72);流速为1.0 ml·min~(-1);检测波长为278 nm。结果:哈巴俄苷进样浓度在0.68～21.92μg·ml~(-1)范围内呈良好的线性关系,r=0.999 9,平均回收率为98.15%,RSD为1.15%(n=6);肉桂酸进样浓度在1.09～35.00μg·ml~(-1)范围内呈良好的线性关系,r=0.999 9,平均回收率为98.42%,RSD为1.14%(n=6)。结论:该方法无干扰,准确、重复性良好,可作为产品的质量控制方法。
Objective: To establish a method for the determination of Harpagoside and cinnamic acid in Shenqi Yangxin Dripping Pills. Methods: The HPLC method was adopted. The column was Kromasil-C 18 column (200 mm × 4.6 mm, 5 μm). The mobile phase consisted of acetonitrile and 1% acetic acid (28:72) and the flow rate was 1.0 ml · min -1 ; Detection wavelength of 278 nm. Results: The concentration of harpagoside was linear in the range of 0.68 ~ 21.92μg · ml ~ (-1), r = 0.999 9, the average recovery was 98.15% and the RSD was 1.15% (n = 6). The concentration of cinnamic acid in the range of 1.09 ~ 35.00μg · ml ~ (-1) showed a good linear relationship, r = 0.999 9, the average recovery was 98.42%, RSD was 1.14% (n = 6). Conclusion: This method has no interference, accuracy and repeatability and can be used as a quality control method.