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目的 探讨急性大鼠脑出血后血脑屏障 (BBB)的损伤及头穴电针对其影响。方法 脑内注血法制备急性脑出血大鼠模型 ,动物分为 4组 :针刺组、模型组、生理盐水组及对照组 ,各组内分为 4个时间点 :给予干预因素后 6h、4d、7d及14d。杀检前采用舌下静脉注射伊文斯蓝 (EB) ,通过脑组织内EB含量评价急性脑出血后BBB的损伤及头穴针刺对其影响。结果 模型组在各时间点与对照组比较差异性极显著 (P <0 0 1) ;针刺组在 6h、4d点与对照组比较差异性极显著 (P <0 0 1) ,在 7d、14d点与模型组比较差异性极显著 (P <0 0 1) ,在 7d点与对照组比较差异性显著 (P <0 0 5 ) ;生理盐水组在各时间点与模型组比较差异性极显著 (P <0 0 1) ,在 6h、4d点与对照组差异性极显著 (P <0 0 1) ,在 7d点与对照组比较差异性显著 (P <0 0 5 )。结论 急性大鼠脑出血后 6h、14d持续BBB损伤 ,头穴针刺治疗脑出血可能与促进BBB损伤的修复有关。
Objective To investigate the damage of blood brain barrier (BBB) and the influence of scalp electroacupuncture (EAA) on the brain after acute intracerebral hemorrhage in rats. Methods The rat models of acute intracerebral hemorrhage were prepared by intracerebral injection of blood. The animals were divided into 4 groups: acupuncture group, model group, saline group and control group. There were 4 time points in each group: 6 h after intervention, 4d, 7d and 14d. Evans blue (EB) was injected sublingually intravenously before the killing, and the damage of BBB after acute intracerebral hemorrhage and the effect of scalp acupuncture on the content of EB in brain tissue were evaluated. Results The difference between the model group and the control group at each time point was significant (P <0.01). There was significant difference between the acupuncture group and the control group at 6h and 4d (P <0.01) At 14d, there was significant difference (P <0.01) between the model group and the model group, and there was significant difference between the model group and the control group at 7d (P <0 05) (P <0.01). There was significant difference (P <0.01) between the control group and the 6h and 4d groups, and significant difference (P <0 05) between the control group and the 7d group. Conclusion The BBB injury sustained at 6h and 14d after acute intracerebral hemorrhage in rats. The treatment of intracerebral hemorrhage by acupuncture with scalp acupuncture may be related to the repair of BBB injury.