论文部分内容阅读
目的采用高压乳匀法将中药有效成分包载于固体脂质纳米粒(SLN),并研究制备的纳米粒的主要性质。方法选择水飞蓟宾(SIL)和汉防己甲素(TET)为模型药物,采用高压乳匀法将其分别包载于SLN。在电镜下观察其形态,以粒度分析仪和Zeta电位分析仪测定其粒径和Zeta电位,用葡聚糖凝胶柱层析法和HPLC测定其包封率和载药量,还观察了SLN的稳定性。结果高压乳匀法制备的SIL-SLN呈球状,形态规则,平均粒径为(157±8)nm,Zeta电位为(-35.36±2.68)mV,包封率为95.64%,载药量为4.63%;TET-SLN呈片状存在,不规则,粒径较小,平均粒径为(47±3)nm,Zeta电位为(-32.99±2.54)mV,包封率为97.82%,载药量为4.76%。SIL-SLN和TET-SLN有较高稳定性。结论高压乳匀法适于制备包载中药的SLN。
Objective To encapsulate the active ingredients of traditional Chinese medicine in solid lipid nanoparticles (SLN) by high-pressure homogenization and to study the main properties of the prepared nanoparticles. Methods Silybin (SIL) and tetrandrine (TET) were chosen as model drugs, and were respectively packaged in SLN by high pressure homogenization method. The morphology was observed under electron microscope. The particle size and Zeta potential were measured by particle size analyzer and Zeta potential analyzer. The entrapment efficiency and drug loading were determined by Sephadex GLC column and HPLC. The SLN Stability. Results The SIL-SLN prepared by high-pressure homogenization method showed a spherical morphology with a mean diameter of 157 ± 8 nm and a Zeta potential of (-35.36 ± 2.68) mV. The encapsulation efficiency was 95.64% and the drug loading was 4.63 %; TET-SLN was flaky, irregular, with a smaller particle size, average particle diameter of (47 ± 3) nm, Zeta potential of -32.99 ± 2.54 mV, encapsulation efficiency of 97.82% 4.76%. SIL-SLN and TET-SLN have higher stability. Conclusion High-pressure homogenization method is suitable for preparation of SLN containing Chinese medicine.