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报道了通过统计学上逐步回归的方法,筛选出对植物耐盐性有显著贡献的蛋白质,并应用方差分析探讨上述蛋白质的最佳诱导条件,以便探明盐胁迫-特定蛋白-耐盐性之间的关系。结果表明:由NaCl诱导的高梁耐盐性是众多蛋白质综合作用的结果,其中不受盐诱导的15.5kD根蛋白、受盐诱导的71.4kD叶蛋白对高梁耐盐性具有显著正贡献;48.9kD根蛋白在盐胁迫下含量有所减少,它的减少对耐盐性有积极作用,还有一些蛋白在盐胁迫下含量有所增加,却与耐盐性无显著相关,甚至对耐盐性有负贡献,其中包括与osmotin表观分子量相同的根蛋白R_(14)。叶片游离脯氨酸含量对高梁耐盐性有正贡献,但不如某些蛋白质的作用显著。
We report that we can find out the proteins that contribute significantly to the salt tolerance of plants through statistical stepwise regression and use variance analysis to explore the optimal conditions for the above proteins in order to find out whether the salt stress-specific protein-salt tolerance Relationship between. The results showed that the salt tolerance of sorghum induced by NaCl was the result of the comprehensive effect of many proteins. Among them, salt-induced 15.5 kD protein, salt-induced 71.4 kD leaf protein had a significant positive contribution to sorghum salt tolerance; 48.9 kD The content of root protein decreased under salt stress, its reduction had a positive effect on salt tolerance, and some proteins increased under salt stress, but no significant correlation with salt tolerance, even salt tolerance Negative contributions include root protein R_ (14) with the same apparent molecular weight as osmotin. Leaf free proline content of sorghum salt has a positive contribution, but not as good as some of the role of protein.