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目的：探讨秋水仙碱（Ｃｏｌ）于在体条件下对重组人肿瘤坏死因子α（ＴＮＦ－α）所致动物肝毒性的影响。方法：将１８只雄性ＢＡＬＢ／ｃ小鼠随机分为Ａ～Ｃ３组，腹腔注射Ｄ－氨基半乳糖（ＧａｌＮ）７００ｍｇ／ｋｇ致敏，１ｈ后再次腹腔注射ＴＮＦ－α５０μｇ／ｋｇ诱发急性肝坏死。Ａ组小鼠于ＴＮＦ－α给药前１９和４ｈ分别腹腔注射生理盐水作为对照，Ｂ和Ｃ组则按相同时间分别灌服或腹腔注射Ｃｏｌ０．５ｍｇ／ｋｇ。给药８ｈ后测定小鼠血清丙氨酸转氨酶（ＡＬＴ）和甘氨酸结合胆汁酸（ＧＣＡ），并观察小鼠肝脏的病理改变。结果：ＴＮＦ－α注射８ｈ后Ｃ组ＡＬＴ及ＧＣＡ水平明显低于Ａ组（Ｐ＜０．０５），而Ｂ组与Ａ组无明显差别；Ｃ组小鼠肝脏病理损害程度亦较Ａ组和Ｂ组明显减轻（Ｐ＜０．０１）。结论：Ｃｏｌ对ＴＮＦα所致的动物肝毒性具有预防作用。
Objective: To investigate the effects of colchicine (Col) on hepatotoxicity induced by recombinant human tumor necrosis factor-α (TNF-α) in vivo. METHODS: Eighteen male BALB/c mice were randomly divided into groups A to C3 and sensitized with intraperitoneal injection of D-galactosamine (GalN) at 700 mg/kg. After 1 h, acute hepatic necrosis was induced by intraperitoneal injection of TNF-α at 50 μg/kg. In group A mice, normal saline was injected intraperitoneally at 19 and 4 hours before TNF-α administration, while group B and C were fed or intraperitoneally injected with Col 0.5 mg/kg at the same time. After 8 hours of administration, serum alanine aminotransferase (ALT) and glycine-conjugated bile acid (GCA) were measured, and the pathological changes of the mouse liver were observed. Results: The levels of ALT and GCA in group C were significantly lower than those in group A (P<0.05) after 8 h injection of TNF-α. There was no significant difference between group B and group A. The degree of liver pathological damage in group C was also higher than that of group A. B group was significantly reduced (P <0.01). Conclusion: Col has a preventive effect on liver toxicity induced by TNFα in animals.