论文部分内容阅读
目的观察异丙酚静脉自身给药对大鼠伏隔核内p-ERK表达的影响。方法 SD大鼠随机分为3组(n=6):对照组(C组)、1.00mg/kg异丙酚组(P1组)、1.70mg/kg异丙酚组(P2组)。用静脉自身给药法建立异丙酚精神依赖模型,Westernblotting法检测大鼠伏隔核内p-ERK和ERK的变化。结果 P1组、P2组的异丙酚可以诱发大鼠建立静脉自身给药行为。与P1组相比,P2组大鼠有效鼻触(P<0.01)和注射次数(P<0.01)明显增加。随着异丙酚剂量的增加,大鼠伏隔核内p-ERK/ERK的表达明显增加(P<0.01)。结论异丙酚静脉自身给药增加了大鼠伏隔核内p-ERK的表达,ERK信号转导通路可能参与了异丙酚的精神依赖性。
Objective To observe the effect of propofol intravenous administration on the expression of p-ERK in the nucleus accumbens in rats. Methods SD rats were randomly divided into 3 groups (n = 6): control group (C group), 1.00 mg / kg propofol group (P1 group) and 1.70 mg / kg propofol group (P2 group). The model of propofol dependence was established by intravenous administration, and the changes of p-ERK and ERK in nucleus accumbens were detected by Western blotting. Results Propofol in group P1 and group P2 induced the self-administration of vein in rats. Compared with P1 group, P2 group rats effective nose touch (P <0.01) and injection times (P <0.01) increased significantly. With the increase of propofol dose, the expression of p-ERK / ERK in nucleus accumbens significantly increased (P <0.01). Conclusion Intravenous self-administration of propofol increases the expression of p-ERK in the nucleus accumbens. ERK signaling pathway may be involved in the neuropsychopharmacological dependence of propofol.