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目的探讨cAMP反应元件调节物(cyclic AMP-responsive element modulator,CREM)与男性不育症中特发性生精障碍的关系。方法收集特发性无精子症患者外周血53例和特发性严重少精子症患者外周血45例,另以具有正常生育能力志愿者外周血30例作为对照,通过提取外周血DNA、PCR扩增、单链构象多态性(SSCP)对外周血中CREM基因进行检测。结果在26.4%的特发性无精子症和25%特发性严重少精子症患者中出现CREM基因的变异。结论在人类,CREM基因与精子的发生发育之间具有相关性,CREM基因参与人类精子的发生。
Objective To investigate the relationship between cAMP response element modulator (CREM) and idiopathic spermatogenic disorders in male infertility. Methods Fifty-three cases of idiopathic azoospermia and 45 cases of idiopathic oligospermia were collected from peripheral blood of 30 cases with idiopathic azoospermia. The other 30 cases of peripheral blood with normal fertility were used as control. Increased, single-strand conformation polymorphism (SSCP) detection of peripheral blood CREM gene. Results A mutation in the CREM gene occurred in 26.4% of idiopathic azoospermia and 25% of idiopathic severe oligospermia patients. Conclusion In humans, there is a correlation between CREM gene and the development of spermatogenesis. CREM gene is involved in the development of human spermatozoa.