论文部分内容阅读
目的:检测口腔鳞癌组织(OSCC)中NBS1的表达水平,探讨其与OSCC病理分化级别的相关性以及在肿瘤发生发展中的作用。方法:①采用免疫组化(SP法)检测NBS1蛋白在30例口腔鳞癌组织、9例癌旁组织中的表达。②应用RT-PCR技术检测组织中NBS1的mRNA表达水平。结果:在OSCC与癌旁正常组织中,NBS1 mRNA和蛋白表达差异显著,在口腔癌组织中均表达较高,并随着病理级别增高而增高(P<0.05)。结论:NBS1在不同病理分化程度OSCC中表达水平的差别与多种因素密切相关,与肿瘤的恶性生物学行为有关,其表达水平可成为判断口腔鳞癌侵袭转移和预后的指标之一。
OBJECTIVE: To detect the expression of NBS1 in oral squamous cell carcinoma (OSCC) and to explore its correlation with pathological grade of OSCC and its role in tumorigenesis. Methods: ① Immunohistochemistry (SP method) was used to detect the expression of NBS1 protein in 30 cases of oral squamous cell carcinoma and 9 cases of paracancerous tissues. ② The mRNA expression level of NBS1 in tissues was detected by RT-PCR. Results: The expressions of NBS1 mRNA and protein were significantly different between OSCC and adjacent normal tissues. The expression of NBS1 mRNA and protein in OSCC was higher than that in OSCC and increased with the pathological grade (P <0.05). CONCLUSION: The difference of expression level of NBS1 in OSCC with different histological grade is closely related to many factors, which is related to the malignant biological behavior of the tumor. The expression level of NBS1 may be one of the indicators to judge the invasion, metastasis and prognosis of OSCC.