本试验以内蒙古地区广泛分布的乌拉尔甘草为试材,研究不同外植体愈伤组织诱导的差异。结果显示,利用MS0培养基接种甘草种子获得了生长健壮的无菌苗,利用无菌苗根尖、茎段、叶片分别诱导愈伤组织,诱导率有较大差异,其中,根尖诱导愈伤组织的诱导率达到77.8%,茎段为52.1%,叶片最低,几乎没有诱导出愈伤组织。以上研究结果为进一步开展甘草有效成分的工业化生产提供了前期实验基础。 In this experiment, Ural licorice widely distributed in Inner Mongolia was used to study the differences of callus induction among different explants. The results showed that inoculation of licorice seed with MS0 medium resulted in the growth of robust sterile seedlings. The callus was induced by root tips, stem segments and leaves of the aseptic seedlings, respectively, Tissue induction rate reached 77.8%, stem segment was 52.1%, leaves the lowest, almost no callus induction. The above results provide a preliminary experimental basis for further industrialization of licorice active ingredients.