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目的探讨对氧磷酶-2(PON2)基因148A/G和311C/S多态性与冠心病(CHD)的关系。方法采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)法对80例CHD患者和160例相匹配的正常人的PON2基因148A/G和311C/S多态性位点进行研究。结果PON2基因148A/G位点AA、AG、GG基因型和A、G等位基因频率在正常对照组和CHD组比较,差异无统计学意义(P>0.05)。PON2基因311C/S位点CC、CS、SS基因型和C、S等位基因频率在两组间比较,差异有统计学意义(P<0.05),CHD组311S/S基因型血浆高密度脂蛋白-胆固醇(HDL-C)水平较正常对照组明显降低(P<0.05),CHD发病率不随两个多态性位点危险基因型个数的增加而呈上升趋势(P>0.05)。结论PON2基因311C/S位点遗传多态性与我国CHD相关联,148A/G和311C/S基因多态性对CHD的影响无协同性。
Objective To investigate the relationship between PON2 148A / G and 311C / S polymorphisms and coronary heart disease (CHD). Methods Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was used to examine the 148A / G and 311C / S polymorphisms of PON2 gene in 80 CHD patients and 160 matched normal subjects . Results The frequencies of AA, AG, GG genotypes and A and G alleles in 148A / G PON2 gene were not significantly different between the normal control group and the CHD group (P> 0.05). The frequencies of CC, CS, SS genotypes and C, S alleles in 311C / S locus of PON2 gene were significantly different between the two groups (P <0.05). The plasma concentrations of high density lipids The level of HDL-C was significantly lower than that of the control group (P <0.05). The incidence of CHD was not increased with the increase of the number of dangerous genotypes at two polymorphic loci (P> 0.05). Conclusion The genetic polymorphism of 311C / S locus in PON2 gene is associated with CHD in China. The polymorphisms of 148A / G and 311C / S have no synergistic effect on CHD.