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采用RT-PCR和RACE技术克隆得到南方鲇(Silurus meridionalis)的CART基因的全长cDNA序列,全长688bp,其中5’非翻译区长109bp,ORF长357bp,3’非翻译区222bp,编码118个氨基酸。与其他脊椎动物的CART蛋白氨基酸序列比对发现,南方鲇CART与斑点叉尾鮰(Ietalurus punetaus)CART、金鱼(Carassius auratus)CART1、金鱼CART2、鲤鱼(Cyprinus carpio)CART1、鲤鱼CART2和人(Homo sapiens)CART同源性分别达到95.8%、72.0%、75.0%、72.9%、75.0%和52.5%,表现出较高的保守性。进化树结果显示,南方鲇的CART与斑点叉尾鮰聚为一支,与基于形态学的鱼类系统发育结论相吻合。组织分布分析表明南方鲇CART基因mRNA主要在脑中表达,与之功能相吻合。研究结果为该种鱼摄食调控机制的研究提供了新的基础资料。
The full-length cDNA sequence of CART gene of Silurus meridionalis was cloned by RT-PCR and RACE technology. The full-length cDNA of CART gene was 688bp in length, including 109 bp in 5 ’untranslated region, 357 bp in ORF and 222 bp in 3’ untranslated region Amino acids. The amino acid sequence alignment of CART proteins from other vertebrates revealed that CART was associated with IART, CART1, Carassius auratus CART1, CART2, Cyprinus carpio CART1, CART2 and Homo sapiens, CART homology reached 95.8%, 72.0%, 75.0%, 72.9%, 75.0% and 52.5%, respectively, showing a high degree of conservation. The results of the phylogenetic tree showed that the CART and the Ictalurus punctatus were clustered together in southern catfish, which was consistent with morphological fish system development. Tissue distribution analysis showed that the mRNA expression of CART mRNA was mainly expressed in the brain, which coincided with its function. The results provide new basic information for the study of regulation mechanism of feeding on fish.