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目的探讨氯化消毒饮用水水样中有机提取物染毒对大鼠肝脏芳香烃受体(aryl hydrcarbon receptor,AhR)和相关基因表达的影响。方法于2012年7—9月(丰水期)采集贵阳市某地水厂的管网末梢水水样并制备有机提取物。将40只清洁级SD大鼠随机分为4组,分别为对照(玉米油)组和5、20、80 L/kg饮用水有机提取物染毒组,每组10只,雌雄各半。采用经口灌胃方式进行染毒,每天1次,连续染毒12周。采用实时荧光定量PCR法检测肝脏中Ah R、热休克蛋白(HSP90)、芳香烃受体核转位蛋白(ARNT)和细胞色素1A2(CYP1A2)mRNA的表达水平,采用Western Blot法检测肝脏中AhR、HSP90、ARNT与CYP1A2蛋白的表达水平。结果与对照组比较,5、20、80 L/kg饮用水有机提取物染毒组大鼠肝脏中HSP90的mRNA和蛋白表达水平均增高,20、80 L/kg组大鼠肝脏中AhR、ARNT与CYP1A2 mRNA和蛋白的表达水平均增高,差异有统计学意义(P<0.05)。结论在本实验条件下,氯化消毒饮用水有机提取物暴露可活化AhR通路。
Objective To investigate the effects of organic extracts from chlorinated disinfection drinking water on the expressions of aryl hydrcarbon receptor (AhR) and related genes in rat liver. Methods From July to September 2012 (wet season), water samples were collected from the pipe network of a waterworks in Guiyang and organic extracts were prepared. Forty clean-grade SD rats were randomly divided into 4 groups: control (corn oil) group and 5,20,80 L / kg drinking water organic extract exposure group, 10 rats in each group. Oral gavage by way of exposure, 1 times a day, continuous exposure to 12 weeks. The expression of Ah R, heat shock protein (HSP90), ARNT and CYP1A2 mRNA in the liver were detected by real-time fluorescence quantitative PCR. The expression of AhR , HSP90, ARNT and CYP1A2 protein expression levels. Results Compared with the control group, the mRNA and protein expressions of HSP90 in the liver of 5, 20, 80 L / kg drinking water organic extract-treated rats increased, while in the 20 and 80 L / kg groups, the contents of AhR and ARNT And CYP1A2 mRNA and protein expression levels were increased, the difference was statistically significant (P <0.05). Conclusion Under this experimental condition, exposure of organic extracts from chlorinated drinking water can activate AhR pathway.