论文部分内容阅读
目的:探讨水飞蓟宾葡甲胺片对大鼠非酒精性脂肪性肝炎的治疗作用及机制。方法:雄性SD大鼠39只,正常喂养1周后随机分为正常对照组(n=9)和造模组(n=30),正常对照组予标准饮食,造模组予高脂饮食。11周末验证造模成功后,将模型动物再分为5组:高脂组(Z组,予高脂饮食及生理盐水ig)、正常饮食组(Y组,予标准饮食及生理盐水ig)、水飞蓟宾葡甲胺片高(A组)、中(B组)及低剂量(C组),剂量分别为100,50,25 mg.kg-1,ig及标准饮食。均连续4周。16周末处死大鼠,检测肝指数、体脂比、血清丙氨酸转移酶(ALT)、天冬氨酸氨基转移酶(AST)、甘油三酯(TC)、总胆固醇(TG)、低密度脂蛋白(LDLC)、血糖(GLU)、超氧化物歧化酶(SOD)、丙二醛(MDA),并行肝组织病理检查及肝匀浆TC,TG,LDLC和MDA测定。结果:第11周末造模组大鼠肝指数、血清AST,TC,TG,LDLC,GLU,MDA水平及肝匀浆TC,TG,MDA水平明显增高,与正常对照组比(P<0.05);治疗组肝组织出现明显脂肪变性、炎症及纤维化(P<0.05)。试验结束时,A组及B组体脂降低;A组血清AST,TC水平明显降低,B组血清MDA水平明显降低(P<0.05);B组肝匀浆TC,LDLC水平明显降低。各治疗组较Y组及Z组肝组织的脂肪变程度、炎症程度和纤维化程度均明显减轻(P<0.05)。结论:水飞蓟宾葡甲胺片可有效治疗大鼠非酒精性脂肪性肝炎。
Objective: To investigate the therapeutic effect and mechanism of silybin meglumine tablets on nonalcoholic steatohepatitis in rats. Methods: Thirty-nine male Sprague-Dawley rats were randomly divided into normal control group (n = 9) and model group (n = 30) one week after normal feeding. The normal control group was given standard diet and the model group was given high fat diet. At the end of 11 weeks, the model animals were successfully subdivided into 5 groups: high fat group (Z group, high fat diet and normal saline ig), normal diet group (Y group, standard diet and saline ig) The silybin tablets were high (group A), medium (group B) and low dose (group C) at dosages of 100, 50 and 25 mg.kg-1, ig and standard diet respectively. All for 4 weeks. The rats were sacrificed on the 16th week, and the liver index, body fat ratio, serum ALT, AST, TC, TG, LDLC, GLU, SOD, MDA, parallel liver histopathological examination and liver homogenate TC, TG, LDLC and MDA were measured. Results: The hepatic index, serum AST, TC, TG, LDLC, GLU and MDA in the model group and the levels of TC, TG and MDA in the liver homogenate were significantly increased at the 11th weekend compared with those in the normal control group (P <0.05). The liver of the treated group showed obvious steatosis, inflammation and fibrosis (P <0.05). At the end of the experiment, body fat decreased in group A and group B; serum AST and TC levels in group A decreased significantly; serum MDA level in group B decreased significantly (P <0.05); TC and LDLC levels in group B decreased significantly. Compared with Y group and Z group, the degree of steatosis, the degree of inflammation and the degree of fibrosis in liver tissue in each treatment group were significantly reduced (P <0.05). Conclusion: Silybin meglumine tablets are effective in treating nonalcoholic steatohepatitis in rats.