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为了解乌梢蛇(Zaocys dhumnades)精子形成的规律,用透射电镜对其头部超微结构进行了观察。结果表明,乌梢蛇精子头部形成可分为4个阶段:阶段Ⅰ,前顶体囊泡内的颗粒物质融合形成1个顶体颗粒而发育为顶体囊泡,随着顶体囊泡的增大,在顶体囊泡与核膜之间形成了致密的纤维物质层。阶段Ⅱ,顶体囊泡变扁平,顶体颗粒分散在顶体囊泡内,前后囊泡膜逐渐相互融合,形成帽状顶体囊。阶段Ⅲ,细胞核离开中心位置移向精子细胞的一极,并在其周围开始有微管聚集,远端中心粒与近端中心粒出现,线粒体和内质网等其他细胞器移向精子细胞的另一极。阶段Ⅳ,细胞核周围汇聚大量微管,随着微管围绕细胞核的直径缩小,细胞核伸长为长卵圆形,同时顶体复合体也发育完成。
To understand the rules of sperm formation in Zaocys dhumnades, the ultrastructure of the head was observed by transmission electron microscopy. The results showed that the head formation of the black snake sperm can be divided into four stages: Stage Ⅰ, the pre-acrosomal vesicles within the particulate material fusion to form a acrosome particles and the development of acrosomal vesicles, with the acrosome vesicles Increases, forming a dense layer of fibrous material between the acrosome vesicles and the nuclear membrane. Stage Ⅱ, acrosomal vesicles become flattened, acrosomal particles dispersed in the acrosome vesicles, before and after the gradual integration of vesicular membrane, forming a cap-like acrosome. Stage Ⅲ, the nucleus moves away from the center to one pole of sperm cells, and begins to gather microtubules around it. The distal centrioles and proximal centrioles appear, and other organelles such as mitochondria and endoplasmic reticulum move to sperm cells. One pole. Stage IV, the convergence of a large number of microtubules around the nucleus, with the microtubules around the nucleus diameter decreases, elongated nucleus oblong, at the same time, acrosome complexes are also developed.