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目的:优化虎杖总游离蒽醌提取工艺。方法:以虎杖总游离蒽醌得率为响应值,在单因素试验基础上,固定提取温度为70℃,提取次数2次,选取溶剂配比、提取时间和液料比3个因素进行Box-Behnken design(BBD)—响应面法(RSM)对其两相酸水解提取工艺进行优化。结果:在提取温度70℃,提取次数2次,溶剂配比0.45,提取时间124 min,液料比26.76时,虎杖总游离蒽醌的提取率最高,可达(16.35±0.75)mg/g。结论:采用Box-Behnken响应面法建立的总游离蒽醌提取工艺模型提取率高,能很好地预测实验结果,优化结果可为生产提供参考依据。
Objective: To optimize the total free anthraquinone extraction process of Polygonum cuspidatum. Methods: The total anthraquinone yield of Polygonum cuspidatum was used as the response value. On the basis of single factor test, fixed extraction temperature was 70 ℃, the number of extraction was 2 times, the ratio of solvent, extraction time, Behnken design (BBD) - Response Surface Methodology (RSM) optimizes its two-phase acid hydrolysis extraction process. Results: The extraction rate of total anthraquinone from Polygonum cuspidatum reached the highest (16.35 ± 0.75) mg / g with extraction temperature of 70 ℃, extraction times of 2, solvent ratio of 0.45, extraction time of 124 min and liquid ratio of 26.76. Conclusion: Box-Behnken response surface method to establish the total free anthraquinone extraction process model extraction rate can be a good prediction of experimental results, the optimization results provide a reference for the production.