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在2-(N-吗啉)乙磺酸(MES)介质中,庆大霉素与纳米金通过Au-N键结合形成复合物,使纳米金聚集,并在543 nm处存在明显共振散射峰,在525 nm和680 nm处存在明显可见吸收峰,据此建立了共振光散射光谱法和比色法检测庆大霉素的方法。在最优实验条件下,庆大霉素浓度在9.31~74.4 nmol/L范围内,543 nm处共振散射光强度增加值(ΔIRLS)与庆大霉素浓度呈现良好的线性关系,其线性回归方程为ΔI=199.5c-131.5(10-8mol/L),r=0.9994。在4.1×10-9~2.2×10~(-5)mol/L浓度范围内时,A_(680)/A_(525)增加值[Δ(A_(680)/A_(525))]也与庆大霉素浓度呈良好的线性关系,其线性回归方程为Δ(A_(680)/A_(5250)=0.21c-0.008(10~(-8)mol/L),r=0.9970。共振光散射法和比色法的样品加标回收率分别为101.6%~102.2%和99.2%~100.4%;相对标准偏差(n=11)分别为2.7%~7.8%和1.9%~7.5%;方法检出限分别为2.79 nmol/L和1.23 nmol/L。方法可用于牛奶中庆大霉素的测定。
In 2- (N-morpholino) ethanesulfonic acid (MES) medium, gentamicin and AuN bond through Au-N bond to form a complex that aggregates AuN and exhibits significant resonance scattering peak at 543 nm , There are obvious visible absorption peaks at 525 nm and 680 nm. Based on this, the methods of detection of gentamycin by resonance light scattering spectroscopy and colorimetric method were established. Under optimal conditions, the concentration of gentamycin was in the range of 9.31-74.4 nmol / L, and the increase of the resonance light scattering intensity (ΔIRLS) at 543 nm showed a good linear relationship with the concentration of gentamicin. The linear regression equation ΔI = 199.5c-131.5 (10-8 mol / L), r = 0.9994. In the concentration range of 4.1 × 10-9 ~ 2.2 × 10 ~ (-5) mol / L, the value of [A (680) / A 525] The linear regression equation was Δ (A 680 / A 5220 = 0.21c-0.008 10 -8 mol / L), r = 0.9970. Resonance light The spiked and colorimetric samples spiked recoveries ranged from 101.6% to 102.2% and 99.2% to 100.4%, respectively. The relative standard deviations (n = 11) were 2.7% -7.8% and 1.9% -7.5%, respectively. The limits were 2.79 nmol / L and 1.23 nmol / L. The method can be used for the determination of gentamicin in milk.