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目的探讨壹期矽肺患者血清中8-异前列腺素F2α(8-iso-PGF2α)、总抗氧化能力(T-AOC)和巨噬细胞衍生趋化因子(MDC)水平变化及意义。方法采用酶联免疫吸附法检测58例壹期矽肺(矽肺组)、35例具有与矽肺组相同接尘条件的健康井下接尘矿工(接尘组)和33例非接尘井上健康人员(对照组)血清中8-iso-PGF2α水平;用Luminex流式荧光技术检测血清MDC水平;化学比色法检测血清T-AOC水平。结果与对照组比较,接尘和矽肺组血清T-AOC水平均明显升高,接尘组MDC水平则明显降低,差异均具有统计学意义(P<0.01);与接尘组比较,矽肺组血清8-iso-PGF2α和T-AOC水平降低,MDC水平升高,差异也具有统计学意义(P<0.05,P<0.01);矽肺并发慢性阻塞性肺疾病(COPD)患者血清T-AOC水平高于单纯矽肺患者(P<0.05);饮酒矽肺患者血清8-iso-PGF2α水平低于非饮酒矽肺患者,差异具有统计学意义(P<0.05);壹期矽肺患者血清T-AOC与MDC呈负相关(r=-0.247,P<0.05)。结论壹期矽肺患者血清8-iso-PGF2α、T-AOC及MDC水平异常,并与矽肺发生发展有关;饮酒可影响壹期矽肺患者血清8-iso-PGF2α水平。
Objective To investigate the changes and significance of 8-iso-PGF2α, T-AOC and MDC in the serum of patients with stage I silicosis. Methods 58 cases of silicosis stage I (silicosis group) were detected by enzyme-linked immunosorbent assay (ELISA), 35 healthy downhole dust miners (dusting group) and 33 healthy non-dusting wells Serum levels of 8-iso-PGF2α were measured. The level of serum MDC was detected by Luminex flow cytometry. The level of serum T-AOC was detected by chemical colorimetry. Results Compared with the control group, serum T-AOC levels in the dust-exposed group and the silicosis group were significantly increased and the MDC level in the dust-exposed group was significantly lower (P <0.01). Compared with the dust-exposed group, Serum levels of 8-iso-PGF2α and T-AOC were lower and MDC levels were higher, the difference was also statistically significant (P <0.05, P <0.01); serum T-AOC levels in patients with silicosis and chronic obstructive pulmonary disease (P <0.05). The level of serum 8-iso-PGF2α in alcoholic silicosis patients was lower than that in non-alcoholic silicosis patients (P <0.05). The serum T-AOC and MDC Negative correlation (r = -0.247, P <0.05). Conclusions Serum levels of 8-iso-PGF2α, T-AOC and MDC in stage I patients with silicosis are abnormal and are related to the development and progression of silicosis. Alcohol consumption may affect the level of serum 8-iso-PGF2α in stage I patients with silicosis.