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目的:探讨17-羟-岩大戟内酯B(HJB)对人脑胶质瘤细胞U251增殖及凋亡的影响。方法:将U251细胞分为空白对照组,5-氟尿嘧啶组(80μmol.L-1),HJB组(6.25,12.5,25,50,100,200,400,800μmol.L-1)。用不同浓度的药物作用24h及药物半数抑制浓度(IC50)浓度作用不同时间(12,24,48,72 h),四甲基偶氮唑蓝(MTT)法检测细胞活性,流式细胞仪Annexin V-FITC/PI检测细胞凋亡率,分光光度法检测半胱氨酸蛋白酶-3(Caspase-3)和半胱氨酸蛋白酶-9(Caspase-9)的相对活性。结果:与空白对照组相比,HJB对U251细胞的增殖有显著抑制作用,并呈浓度依赖及时间依赖性(P<0.05),作用24 h后IC50为62.236 11μmol.L-1。HJB可诱导U251细胞凋亡,浓度30,60,120μmol.L-1分别处理细胞24 h后,早期凋亡率明显升高(P<0.05),且呈浓度依赖关系;Caspase-3及Caspase-9的相对活性均升高(P<0.05),并呈浓度依赖关系。结论:HJB明显抑制体外U251细胞生长并诱导其凋亡,线粒体途径可能是诱导其凋亡的机制之一。
AIM: To investigate the effects of 17-hydroxy-petridrel B (HJB) on the proliferation and apoptosis of human glioma U251 cells. Methods: U251 cells were divided into blank control group, 5-fluorouracil group (80μmol.L-1) and HJB group (6.25,12.5,25,50,100,200,400,800μmol.L-1). The cells were treated with different concentrations of drugs for 24 h and IC50 concentration for different times (12,24,48 and 72 h), MTT assay and flow cytometry Annexin V-FITC / PI was used to detect the apoptosis rate. The relative activity of caspase-3 and Caspase-9 was detected by spectrophotometry. Results: Compared with the blank control group, HJB had a significant inhibitory effect on the proliferation of U251 cells in a concentration-dependent and time-dependent manner (P <0.05). IC50 was 62.236 11 μmol.L-1 after 24 h. HJB could induce the apoptosis of U251 cells. The apoptosis rate of U251 cells treated with 30, 60 and 120μmol.L-1 for 24 h were significantly increased (P <0.05), and the concentration of Caspase-3 and Caspase-9 The relative activity were increased (P <0.05), and in a concentration-dependent manner. Conclusion: HJB significantly inhibits the growth of U251 cells and induces apoptosis in vitro. Mitochondrial pathway may be one of the mechanisms of apoptosis induced by HJB.