Objective:To investigate the effect of MUC2 antisense oligodeoxynucleotide(ASODN)on cell proliferation,adhesion and proteolytic enzyme in human gastric carcinoma cell line(SGC7901).Methods:Phosphorothioate MUC2 ASODN was synthesized and packaged by lipofectin,and then transfected to SGC7901 cells.The expression of MUC2 mRNA and protein after transfection was detected by reverse transcription-polymerase chain reaction(RT-PCR)and immunohistochemical method respectively,and the effect of MUC2 ASODN on cell proliferation,adhesion and proteolytic enzyme was determined by flow cytometry(FCM),MTT method,Rose Bengal and immunohistochemical method.Results:Compared with the blank control group,ASODN efficiently downregulated the expression of MUC2 mRNA and protein in SGC7901 cells 48h after transfection(P<0.01).Various concentrations of ASODN could significantly inhibit the growth of SGC7901,and the inhibition peaked at the 48th hour after transfection(P<0.05).The apoptosis rate of the experimental group was about 4.38%,and the percentage of S-phase cells rose while G0/G1-phase cells fell because most of them were blocked at S-phase.In addition,cells treated with MUC2 ASODN showed lower adhesion ability with matrix and endothelial cells than control cells in vitro(P<0.01).By immunohistochemical method,the upregulation of E-cadherin proteins and the downregulation of MMP2 and cathepsinD proteins were also observed(P<0.05).Conclusion:MUC2 ASODN could efficiently inhibit SGC7901 cell proliferation,reduce cell adhesion ability and downregulate the expression levels of proteolytic enzyme in vitro. Objective: To investigate the effect of MUC2 antisense oligodeoxynucleotide (ASODN) on cell proliferation, adhesion and proteolytic enzyme in human gastric carcinoma cell line (SGC7901). Methods: Phosphorothioate MUC2 ASODN was synthesized and packaged by lipofectin, and then transfected to SGC7901 cells. The expression of MUC2 mRNA and protein after transfection was detected by reverse transcription-polymerase chain reaction (RT-PCR) and immunohistochemical method respectively, and the effect of MUC2 ASODN on cell proliferation, adhesion and proteolytic enzyme was determined by flow cytometry (FCM) , MTT method, Rose Bengal and immunohistochemical method. Results: Compared with the blank control group, ASODN efficiently downregulated the expression of MUC2 mRNA and protein in SGC7901 cells 48h after transfection (P <0.01) .Various concentrations of ASODN could significantly inhibit the growth of SGC7901, and the inhibition peaked at the 48th hour after transfection (P <0.05). The apoptosis rate of the experimen tal group was about 4.38%, and the percentage of S-phase cells rose while G0 / G1-phase cells fell because most of them were blocked at S-phase. In addition, cells treated with MUC2 ASODN showed lower adhesion ability with matrix and (P <0.01) .By immunohistochemical method, the upregulation of E-cadherin proteins and the downregulation of MMP2 and cathepsin D proteins were also observed (P <0.05) .Conclusion: MUC2 ASODN could efficiently inhibit SGC7901 cell proliferation, reduce cell adhesion ability and downregulate the expression levels of proteolytic enzyme in vitro.