论文部分内容阅读
采用RT-PCR的方法,根据栽培大豆的RNF12基因全长设计特异引物,从野生大豆克隆到GsRNF12基因。序列分析表明,该基因含723 bp的开放阅读框(ORF),编码240个氨基酸,GsRNF12蛋白在178~220氨基酸处有典型的C4HC3结构域,属于C3HC4锌指蛋白家族。利用实时荧光定量PCR对野生大豆GsRNF12基因的表达特性进行分析,结果表明:野生大豆GsRNF12基因对高盐、干旱、低温、ABA、SA胁迫处理均存在不同程度的应答响应;GsRNF12基因在根、茎、叶的表达有差异性,其中在根中表达量最大。
Using RT-PCR method, specific primers were designed according to the full-length of RNF12 gene in cultivated soybean, and the GsRNF12 gene was cloned from wild soybean. Sequence analysis showed that the gene contained a 723 bp open reading frame (ORF) encoding 240 amino acids. The GsRNF12 protein has a typical C4HC3 domain from 178 to 220 amino acids and belongs to the C3HC4 zinc finger protein family. The expression characteristics of GsRNF12 gene in wild soybean were analyzed by real-time fluorescence quantitative PCR. The results showed that GsRNF12 gene of wild soybean responded differently to high salt stress, drought stress, low temperature stress, ABA stress and SA stress. , The expression of leaves is different, of which the maximum expression in the root.