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从山西运城的南瓜(Cucurbita moschata)、河南开封和孟津的西葫芦(Cucurbita pepo)上通过ELISA分别检测到南瓜花叶病毒(Squash mosaic virus,SqMV)、西瓜花叶病毒(Watermelon mosaic virus,WMV)和番木瓜环斑病毒西瓜株系(Papa-ya ringspot virus-Watermelon type,PRSV-W),通过接种分离纯化获得其病毒毒原(CH99/211,CH99/113,CH99/69)。本研究克隆了这3个分离物的外壳蛋白基因,应用DNAStar、Clustal X、Phylip等分析软件将这些序列同已报道的相应序列进行分析并构建系统树。结果发现SqMV可以分为两个组,CH99/211分离物与美国的Arizona分离物接近,应属于Z组;WMV分为两个组,CH99/69同国内黑龙江分离物(WMV-HLJ)和云南分离物(WMV-CHN)最接近,成一小簇;PRSV分成两个组,CH99/113属于Ⅱ组,与国内SP、MZ和BN分离物接近。序列分析表明PRSV和WMV在进化上具有地理相关性。SqMV和PRSV-W外壳蛋白基因序列分析属国内首次报道。
Squash mosaic virus (SqMV), Watermelon mosaic virus (WMV), and watermelon mosaic virus (WMV) were detected by ELISA from Cucurbita moschata in Yuncheng, Henan and Cucurbita pepo in Mengjin, The papaya ringspot virus-Watermelon type (PRSV-W) was isolated and purified by inoculation to obtain its virulence (CH99 / 211, CH99 / 113, CH99 / 69). In this study, the coat protein genes of these three isolates were cloned and analyzed by using DNAStar, Clustal X, Phylip and other analysis software to construct the phylogenetic tree. The results showed that SqMV could be divided into two groups. The CH99 / 211 isolates were close to the Arizona isolates in the United States and belonged to the Z group. WMV was divided into two groups. The CH99 / 69 and the domestic Heilongjiang isolates (WMV-HLJ) The isolates (WMV-CHN) were closest to each other and became a small cluster. PRSV was divided into two groups. CH99 / 113 belonged to group II, which was close to the domestic SP, MZ and BN isolates. Sequence analysis showed that PRSV and WMV are evolutionarily related. SqMV and PRSV-W coat protein gene sequence analysis is the first report in China.