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从细胞水平和动物模型两个层次上研究了负载紫杉醇的聚乳酸纤维毡诱导U14宫颈癌细胞凋亡和抑制小鼠U14皮下移植瘤生长的能力.将U14细胞在纤维毡存在下孵育48 h,经Annexin V-FITC及PI双染后行流式细胞分析.结果表明,载药纤维(折合紫杉醇浓度40)g/mL)组总凋亡细胞比例(25.6%)明显高于对照组(1.0%)和未载药纤维组(1.5%).建立U14宫颈癌皮下移植瘤小鼠模型,将其随机分为3组.A组为对照组,不做任何处理.B、C组小鼠以纳米纤维毡覆盖于肿瘤表面,覆盖率约为70%～75%.其中B组纤维毡为纳米聚乳酸电纺丝纤维,不载药,C组为同种材料纤维毡,载有33 wt%紫杉醇.经处理后第7、14天每组各处死动物5～7只,剥离肿瘤,照相,称重,计算抑瘤率.结果表明,载药纤维对U14宫颈癌皮下移植瘤有明显抑制作用(48%～56%).用未载药聚乳酸纤维包裹肿瘤表面,肿瘤质量与对照组无显著差别,说明聚乳酸纤维本身对肿瘤没有抑制作用,载药纤维组所观察到的抑瘤效果为紫杉醇从纤维毡中释放所致.
From the cellular level and the animal model, we studied the ability of paclitaxel-loaded polylactic acid fiber mats to induce the apoptosis of U14 cervical cancer cells and inhibit the growth of mouse U14 subcutaneous xenografts.U14 cells were incubated in the presence of fiber mat for 48 h, Flow cytometry analysis of Annexin V-FITC and PI double staining showed that the percentage of total apoptotic cells (25.6%) was significantly higher than that of the control group (1.0%) after loading fiber (equivalent to paclitaxel concentration 40) g / mL) ) And no drug-loaded fiber group (1.5%) .A mouse model of U14 cervical cancer subcutaneously transplanted tumor was established and randomly divided into three groups.A group as the control group, without any treatment.B group, C mice with nano Fibrous mats covered the tumor surface with the coverage rate of about 70% -75%, among which, group B mats were nano-polylactic acid electrospun fibers and did not carry drugs. Group C was the same type of mats and contained 33 wt% paclitaxel After the treatment, on days 7 and 14, 5 to 7 animals were sacrificed in each group, and the tumors were dissected, photographed and weighed to calculate the tumor inhibition rate. The results showed that the drug-loaded fibers significantly inhibited the subcutaneous xenografts of U14 cervical cancer 48% ~ 56%) .Using drug-loaded polylactic acid fiber wrapped tumor surface, the tumor mass and the control group no significant difference, indicating that the polylactic acid fiber There was no inhibitory effect on the tumor, and the anti-tumor effect observed in the drug-loaded fiber group was due to the release of paclitaxel from the fiber mat.