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为探讨耳石碳(δ~(13)C)、氧(δ~(18)O)稳定同位素在淡水鱼类群体识别中的作用,本研究以养殖条件下不同年龄组四川裂腹鱼为对象,采用稳定同位素质谱仪进行碳、氧同位素测定,揭示耳石中碳、氧稳定同位素特征,探讨其与环境间的关系.结果表明:1+龄四川裂腹鱼δ~(13)C和δ~(18)O值均与耳石质量无显著相关关系,但在微耳石和星耳石之间存在显著差异;不同年龄四川裂腹鱼微耳石δ~(13)C和δ~(18)O平均值分别为(-9.58±0.06)‰、(-8.33±0.17)‰,其在雌雄个体之间均无显著性差异,但在不同年龄组间存在显著差异.耳石δ~(18)O和δ~(13)C的关联分析能有效区分四川裂腹鱼不同养殖年龄群体,可作为一种识别淡水鱼类养殖群体的手段.
To investigate the role of otolith carbon (δ ~ (13) C) and oxygen (δ ~ (18) O) stable isotopes in the freshwater fish population identification, the present study aimed to investigate the effects of otolith carbon (δ 13 C) , Stable isotope mass spectrometer carbon and oxygen isotope determination, to reveal the characteristics of carbon and oxygen stable isotopes of otoliths, to explore the relationship between the environment and the environment.The results showed that: 1 + Sichuan Crayfish fish δ ~ (13) C and δ There was no significant correlation between ~ (18) O value and otolith quality, but there was significant difference between otolith and otolith; δ ~ (13) C and δ ~ 18 ) O were (-9.58 ± 0.06) ‰ and (- 8.33 ± 0.17) ‰, respectively, and there was no significant difference between male and female individuals, but there were significant differences among different age groups. ) The correlation analysis of O and δ ~ (13) C can effectively distinguish different populations of Crassostrea nigra in Sichuan, which can be used as a means of identifying freshwater fish farming population.