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目的探讨产前应用不同疗程糖皮质激素(GCs)对仔鼠海马鸟苷酸脱氢酶(ODC)活性的影响。方法健康3月龄雌性SD大鼠30只,孕18d时(E18)随机分为3组:多疗程组,E18开始每天肌注地塞米松0.48mg/(kg.次),q4h,4次/d,连用3d;单疗程组,E18肌注地塞米松0.48mg/(kg.次),q4h,4次为1个疗效,共1个疗程,余2d以等容积9g/L盐水代替;对照组以等容积9g/L盐水代替。于出生第3、15天(P3、P15)取仔鼠脑组织采用免疫组织化学法测定其海马部位ODC酶的活性。结果免疫组织化学显示P3时仔鼠海马部位ODC酶的活性在各组均有表达,其COD值分别为5.78±1.62、10.37±1.36、11.96±1.13,但多疗程组较单疗程组、对照组明显降低(Pa<0.01);P15多疗程组仔鼠海马部位ODC活性明显增高,单疗程组明显降低,对照组几乎消失,其COD值分别为12.47±1.52、6.73±1.08、5.12±0.78;P3、P15单疗程组与对照组间比较均无显著差别(Pa>0.05)。结论产前多疗程应用GCs可导致仔鼠脑组织海马部位ODC活性表达的改变,从而影响海马的功能,如学习记忆能力;临床上对高危早产孕妇产前重复应用GCs需权衡利弊。
Objective To investigate the effect of prenatal application of different courses of glucocorticoid (GCs) on the activity of guanylate dehydrogenase (ODC) in hippocampus of offspring. Methods Thirty healthy three-month-old female Sprague-Dawley rats were randomly divided into three groups at the age of 18 days (E18): multiple courses of treatment, dexamethasone 0.48 mg / (kg) d, continuous use of 3d; single treatment group, E18 intramuscular injection of dexamethasone 0.48mg / (kg. times), q4h, 4 times for the 1 effect, a total of 1 course of treatment, the remaining 2d with an equal volume of 9g / L saline instead; control Group with an equal volume of 9g / L saline instead. On day 3 and day 15 of birth (P3 and P15), the brain tissue of offspring rats were harvested and the ODC enzyme activity of hippocampus was measured by immunohistochemistry. Results Immunohistochemistry showed that the activity of ODC enzyme in hippocampus of P3 rats was expressed in all groups, with COD values of 5.78 ± 1.62, 10.37 ± 1.36 and 11.96 ± 1.13, respectively. However, compared with single treatment group and control group (P <0.01). The ODC activity in the hippocampus of the offspring of P15 multi-treatment group was significantly higher than that in the single treatment group, and disappeared in the control group. The COD values were 12.47 ± 1.52,6.73 ± 1.08 and 5.12 ± 0.78, respectively. P3 , P15 single treatment group and the control group were no significant difference (Pa> 0.05). Conclusions GCs can increase the changes of the ODC activity in the hippocampus of the brains of the offspring, which may affect the function of the hippocampus, such as learning and memory ability. GCs should be weighed in the prenatal treatment for the pregnant women with high-risk pre-term pregnancy.