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建立了扶骨黄酮分散片中的葛根素、大豆苷和淫羊藿苷含量测定的高效液相色谱法。采用Eclipse XDB-C18(4.6 mm×250 mm,5μm)色谱柱,流动相为乙腈:0.3%乙酸水溶液梯度洗脱,流速为0.8 m L/min,柱温为25℃,葛根素、大豆苷检测波长为250 nm,淫羊藿苷检测波长为270 nm。结果表明,葛根素检测浓度在0.002 08 mg/m L~0.104 mg/m L范围内与峰面积线性关系良好,加样回收率为100.29%,相对标准偏差为0.89%;大豆苷检测浓度在0.000 98 mg/m L~0.039 2 mg/m L范围内与峰面积线性关系良好,加样回收率为98.29%,相对标准偏差为1.29%;淫羊藿苷检测浓度在0.001 04 mg/m L~0.041 6 mg/m L范围内与峰面积线性关系良好,加样回收率为99.07%,相对标准偏差为1.08%。该方法简便、准确,重现性和稳定性好,可作为扶骨黄酮分散片的质量控制标准。
A HPLC method was developed for the determination of puerarin, daidzin and icariin. The mobile phase consisted of a gradient elution of acetonitrile: 0.3% acetic acid with a flow rate of 0.8 m L / min and a column temperature of 25 ℃ using Eclipse XDB-C18 (4.6 mm × 250 mm, 5 μm) Wavelength of 250 nm, icariin detection wavelength of 270 nm. The results showed that the calibration curve of puerarin showed a good linear relationship with the peak area in the range of 0.002 08 mg / m L to 0.104 mg / m L with a recovery of 100.29% and a relative standard deviation of 0.89%. Daidzein concentration was 0.000 The linear relationship between the peak area and the range of 98 mg / m L to 0.039 2 mg / m L was good with the recovery of 98.29% and the relative standard deviation of 1.29%. The detection concentrations of icariin were between 0.001 04 mg / m L ~ The linear range of the peak area was 0.041 6 mg / m L, with the recovery of 99.07% and the relative standard deviation of 1.08%. The method is simple, accurate, reproducible and stable, and can be used as a quality control standard for the compound of radix isatidis dispersible tablets.