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用各种不同培养基对马铃薯四倍体、双单倍体栽培种及有结薯习性的野生种进行了试管微型薯诱导。在其它条件不变时,用食用白糖替代蔗糖可以获得同样的诱导效果,能大大降低成本。液体培养较固体培养效果好。BAP 的诱导效果随基因型不同而异,但并不一定是必需的。香兰素补加到液体培养基中对提高诱导效果有一定作用。试验表明用 MS 加10%食用白糖或 MS 加10%白糖再补加一定浓度香兰素的液体培养基,在黑暗条件下室温于20～28℃培养是高效率生产试管微型薯较好的方法。
In vitro culture of potato tetraploid, double haploid cultivars and wild tubers with tuberous habits were conducted in vitro and induced in vitro. In other conditions remain unchanged, the replacement of sucrose with edible sugar can get the same induction effect, can greatly reduce costs. Liquid culture better than solid culture. The induction effect of BAP varies with the genotype, but it is not always necessary. The addition of vanillin to the liquid medium has a certain effect on improving the induction effect. Tests showed that MS plus 10% edible sugar or MS plus 10% sugar and then add a certain concentration of vanillin liquid medium at room temperature under dark conditions at 20 ~ 28 ℃ is a good method for the efficient production of test tube microtubes .